Collaborative Research: Nanoscale Cues for Regenerative Neural Cell Systems

合作研究：再生神经细胞系统的纳米级线索

• 批准号: 0957776
• 负责人: Virginia Ayres
• 金额: $ 34.99万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-10-01 至 2015-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0957776&HistoricalAwards=false
• 关键词: Collaborative; Research; Nanoscale; Cues; Regenerative

In this project the PI will investigate the hypothesis that there is a fundamental set of nanoscale physical and biochemical properties that serve as the primary cues for the re-establishment of cell systems that rely on nanofibrillar scaffolds in their native environment, and they will use an integrated approach for their investigation. A novel investigative tool, scanning probe recognition microscopy, will be used in concert with state of the art immunocytochemistry, fluorescent microscopy and blot techniques in an integrated physics-cell biology effort that links the physical properties at the nanoscale with important cellular responses. Results from this research are likely to have a major impact on regenerative medicine aimed at recreating the neural cell system following central nervous system injury. Furthermore, as cell systems that are influenced by scaffold-based environments are ubiquitous throughout the body, identification of the fundamental set of nanoscale properties that promote healthy neural cell physiology and function, serves as the basis for the quantitative exploration of other regenerative cell-scaffold systems. The research program will be realized with active student participation. Regular exchange visits, joint training, and regular communication, with evaluation and dissemination of results, will contribute to the multidisciplinary training. The proposed pipeline program for the introduction of traditionally underrepresented undergraduates within the research, in collaboration with the mentored MI-LSAMP and RISE programs, will build on the PI,s successful experiences with both.

在这个项目中，PI 将研究这样一个假设：存在一组基本的纳米级物理和生化特性，这些特性可作为在原生环境中重建依赖于纳米纤丝支架的细胞系统的主要线索，并且他们将使用综合方法进行研究。一种新颖的研究工具，即扫描探针识别显微镜，将与最先进的免疫细胞化学、荧光显微镜和印迹技术一起用于物理-细胞生物学的综合工作，将纳米级的物理特性与重要的细胞反应联系起来。这项研究的结果可能会对再生医学产生重大影响，再生医学旨在重建中枢神经系统损伤后的神经细胞系统。此外，由于受支架环境影响的细胞系统在整个身体中无处不在，因此识别促进健康神经细胞生理和功能的基本纳米级特性，可以作为定量探索其他再生细胞支架系统的基础。该研究计划将在学生的积极参与下实现。定期互访、联合培训、定期沟通，评估和传播成果，有利于多学科培训。拟议的管道计划将与受指导的 MI-LSAMP 和 RISE 计划合作，在研究中引入传统上代表性不足的本科生，该计划将建立在 PI 在这两个项目上的成功经验的基础上。