Collaborative Research: Investigating and Improving the Production of Butanol by C. Pasteurianum for the Value-Added Conversion of Biodiesel-Derived Crude Glycerol

合作研究：研究和改进巴氏梭菌生产丁醇，用于生物柴油衍生的粗甘油的增值转化

• 批准号: 0966818
• 负责人: Geoffrey Bothun
• 金额: $ 13.44万
• 依托单位: University of Rhode Island
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0966818&HistoricalAwards=false
• 关键词: Collaborative; Research; Investigating; Improving; Production

0966846/0966818Taconi/BothunAs the price of crude oil continues to reach record highs, biorefining becomes a necessary option for renewable fuels and chemical production. However, robust and versatile microorganisms for bioconversion that can utilize multiple feedstocks are currently lacking. Better engineered microorganisms designed to function within an integrated biorefining system are required to significantly reduce petroleum utilization and develop a diverse portfolio of biomass-based processes that can generate comparable alternatives for a wide range of petroleum products.Utilization of biodiesel-derived crude glycerol for the production of butanol represents a tangible approach to improving the sustainability of the biofuels industry in general and is critical for the growth and stability of the biodiesel industry in particular. Furthermore, utilizing crude glycerol as an alternative to corn-derived feedstocks will provide much-needed diversity and flexibility to an emerging biorefining industry limited by the price and availability of corn. Butanol is a key biorefinery platform chemical and has significant advantages as a renewable transportation fuel. The potential to generate higher yields using an essentially no-cost feedstock strengthens the technical and economic feasibility for industrial-scale butanol fermentation.Intellectual MeritThe goal of this research is to quantify metabolism and tolerance in Clostridium pasteurianum and to engineer this bacterium to improve the value-added conversion of biodiesel-derived crude glycerol using anaerobic fermentation. This project will focus on fundamental research to understand and improve the metabolic pathways that control glycerol utilization and substrate formation from both extracellular and intracellular approaches. The proposed research has three objectives. The first objective is toelucidate the cellular response of C. pasteurianum to both substrate and product toxicity. The second objective is to evaluate cell membrane structure and stability in response to increasing concentrations of substrate impurities and butanol. And the third objective is to metabolically engineer C. pasteurianum to increase butanol production.The research is innovative because it proposes an alternative pathway to glycerol fermentation, where metabolic engineering approaches are used to knock down the genes in the reductive pathway as opposed to up-regulation of genes in the oxidative pathway. The research is potentially transformative because it opens up new approaches for metabolic engineering of microorganisms to convert crude glycerol byproducts from biorefinery operations to liquid transportation fuels.Broader ImpactsThis research project provides opportunities to educate students and other educators about alternative energy, renewable resources, and biorefining. The education plan will develop the technical and laboratory expertise for graduate and undergraduate students to prepare them for careers in the biofuels industry, which is currently experiencing substantial growth. Various outreach activities are planned, including the development of a seminars and demonstrations to educate K-12 students, teachers, and community members about biofuels production and utilization. Education efforts will also focus on the recruitment of students from underrepresented groups through activities that will target the inclusion of these students. These educational and outreach activities will stimulate broad interest in science and engineering, and inspire K-12 students to pursue higher education and careers in engineering and energy industries. The proposed outreach activities also include a web based wiki site and summer exchange program that will facilitate collaboration among graduate and undergraduate students in the two research groups.

0966846/0966818TACONI/BOTHUNAS原油的价格继续达到创纪录的高点，生物修饰成为可再生燃料和化学生产的必要选择。 但是，目前缺乏用于使用多个原料的生物转化的强大而多功能的微生物。 旨在在集成的生物修补系统中发挥作用的更好的工程微生物需要显着降低石油利用，并开发出多种基于生物量的过程的组合，这些过程可以为多种石油化生成可比的替代品，以改善生物燃料源的butanol的生产，以改善伊斯兰的生产，以改善伊斯兰的生产。尤其是生物柴油行业的增长和稳定性。此外，利用粗甘油作为玉米衍生的原料的替代品将提供急需的多样性和灵活性，以限制玉米的价格和可用性的新兴生物改造行业。丁醇是一种关键的生物融资平台化学化学，作为可再生运输燃料具有显着优势。 The potential to generate higher yields using an essentially no-cost feedstock strengthens the technical and economic feasibility for industrial-scale butanol fermentation.Intellectual MeritThe goal of this research is to quantify metabolism and tolerance in Clostridium pasteurianum and to engineer this bacterium to improve the value-added conversion of biodiesel-derived crude glycerol using anaerobic fermentation. 该项目将着重于基础研究，以了解和改善从细胞外和细胞内方法控制甘油利用和底物形成的代谢途径。 拟议的研究有三个目标。 第一个目的是促降义硫酸梭菌对底物和产物毒性的细胞反应。 第二个目标是评估细胞膜结构和稳定性，以响应增加的底物杂质和丁醇的浓度。 第三个目标是用代谢工程师的抗胸腔念珠菌来增加丁醇的产生。这项研究具有创新性，因为它提出了一种替代甘油发酵的途径，在这种途径中，使用代谢工程方法来击倒还原途径中与氧化途径中基因上调相反的基因。 这项研究具有潜在的变革性，因为它为微生物的代谢工程打开了新的方法，以将原油甘油副产品从生物填充业务转变为液体运输燃料。这项研究项目提供了机会，为学生和其他教育者提供有关替代能源，可再生资源和生物修复的教育机会。 该教育计划将为研究生和本科生开发技术和实验室专业知识，以为生物燃料行业的职业做好准备，该行业目前正在经历大幅增长。 计划进行各种外展活动，包括开展研讨会和示威活动，以教育K-12学生，老师和社区成员有关生物燃料的生产和利用。 教育工作还将集中于通过针对这些学生的活动的活动来招募来自代表性不足的群体的学生。这些教育和外展活动将激发人们对科学和工程的广泛兴趣，并激发K-12学生从事工程和能源行业的高等教育和职业。拟议的外展活动还包括一个基于Web的Wiki网站和夏季交流计划，该计划将促进两个研究小组的研究生和本科生之间的合作。