In vivo dissection of functional domains of plant glutamyl-tRNA reductase, the regulatory key enzyme of tetrapyrrole biosynthesis

四吡咯生物合成的关键调节酶植物谷氨酰-tRNA还原酶功能域的体内解剖

基本信息

项目摘要

Glutamyl-tRNA reductase (GluTR), the first committed enzyme of plant tetrapyrrole biosynthesis and considered to be the rate limiting enzyme for the supply of 5-aminolevulinic acid (ALA), is encoded in dicotyledonous plants by two HEMA genes. Posttranslational mechanisms control GluTR activity and interactions have been described with heme, FLU and GBP, a newly identified GluTR-binding protein with potential to recruit enzymes for ALA synthesis dedicated to heme biosynthesis. Previous in vitro and yeast analyses revealed domains for heme- and FLU-binding of plant GluTR. It is the aim of the proposal to dissect the functional domains of GluTR interacting with regulatory factors in Arabidopsis in vivo. Modified GluTRs are expressed in the hemA1 mutant and analysed for the degree of regulatory complementation of ALA and tetrapyrrole biosynthesis. It is proposed that the two GluTR isoforms differentially interact with their effectors, thereby forming different protein complexes for ALA synthesis which facilitate specific contributions to chlorophyll or heme biosynthesis. Structural features determining functional differences will be dissected by domain swapping experiments, while biochemical and genetic approaches aim at the characterization of ALA-synthesizing protein complexes containing GluTR1 and GluTR2, respectively. Evolution of posttranslational regulation in plant GluTR is addressed by starting interaction studies engaging further dicotyledonous plant species.
谷氨酰-tRNA还原酶(Glutamyl-tRNA reductase,GluTR)是植物四吡咯合成的第一个关键酶,是5-氨基乙酰丙酸(5-aminolevulinic acid,ALA)合成的限速酶。翻译后机制控制GluTR活性和相互作用已被描述与血红素,FLU和GBP,一个新发现的GluTR结合蛋白,有可能招募酶的ALA合成,专门用于血红素生物合成。先前的体外和酵母分析揭示了植物GluTR的血红素和FLU-binding域。这是该建议的目的是解剖的功能结构域的GluTR与调节因子在拟南芥体内相互作用。修饰的GluTRs在hemA 1突变体中表达,并分析ALA和四吡咯生物合成的调节互补程度。有人提出,这两个GluTR异构体差异与它们的效应相互作用,从而形成不同的蛋白质复合物ALA合成,促进叶绿素或血红素生物合成的具体贡献。决定功能差异的结构特征将通过结构域交换实验进行解剖,而生化和遗传方法的目的是分别表征含有GluTR 1和GluTR 2的ALA合成蛋白质复合物。植物GluTR的翻译后调控的进化是通过进一步开展双子叶植物物种的相互作用研究来解决的。

项目成果

期刊论文数量(1)
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会议论文数量(0)
专利数量(0)

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Professor Dr. Bernhard Grimm其他文献

Professor Dr. Bernhard Grimm的其他文献

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{{ truncateString('Professor Dr. Bernhard Grimm', 18)}}的其他基金

Identification and analysis of genes involved in tetrapyrrole metabolism and plastid-derived ROS-mediated signaling in Chlamydomonas reinhardtii
莱茵衣藻中参与四吡咯代谢和质体衍生的 ROS 介导信号传导的基因的鉴定和分析
  • 批准号:
    290763515
  • 财政年份:
    2016
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Auxiliary factors for chlorophyll biosynthesis and their potential contribution to the assembly of chlorophyll into chlorophyll-binding proteins
叶绿素生物合成的辅助因子及其对叶绿素组装成叶绿素结合蛋白的潜在贡献
  • 批准号:
    254831670
  • 财政年份:
    2014
  • 资助金额:
    --
  • 项目类别:
    Research Units
In-planta analyses of the three Arabidopsis isoforms of the bifunctional RIBA with enzymatic domains for GTP cyclohydrolase II and 3,4-dihydroxy-2-butanone 4-phosphate synthase
对具有 GTP 环化水解酶 II 和 3,4-二羟基-2-丁酮 4-磷酸合酶酶结构域的双功能 RIBA 的三种拟南芥亚型进行植物内分析
  • 批准号:
    258667322
  • 财政年份:
    2014
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Thiol-based Redox Control of Tetrapyrrole Metabolism: Posttranslational Control of Tetrapyrrole Biosynthesis Enzymes by NADPH-Dependent Thioredoxin Reductase C (NTRC) and Thioredoxins
四吡咯代谢的基于硫醇的氧化还原控制:NADPH 依赖性硫氧还蛋白还原酶 C (NTRC) 和硫氧还蛋白对四吡咯生物合成酶的翻译后控制
  • 批准号:
    251911153
  • 财政年份:
    2014
  • 资助金额:
    --
  • 项目类别:
    Priority Programmes
Identification and analysis of Chlamydomonas mutants with defects in chlorophyll synthesis
叶绿素合成缺陷衣藻突变体的鉴定与分析
  • 批准号:
    98772029
  • 财政年份:
    2008
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Dissection of the tetrapyrrole-mediated signaling pathway: Identification of components involved in signal transduction and of target genes
剖析四吡咯介导的信号通路:鉴定参与信号转导的成分和靶基因
  • 批准号:
    42287343
  • 财政年份:
    2007
  • 资助金额:
    --
  • 项目类别:
    Research Units
Central Task
中心任务
  • 批准号:
    42308303
  • 财政年份:
    2007
  • 资助金额:
    --
  • 项目类别:
    Research Units
Untersuchungen zur Regulation der am Chlorophyll-Abbauweg beteiligten Enzyme
叶绿素降解途径相关酶的调控研究
  • 批准号:
    5456693
  • 财政年份:
    2005
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Functional analysis of the NF-Y transcription factor family for gene activation during adaptation to environmental stress. Exploration of properties and functions of specific members of the NF-Y family for gene activation during adaptation to environmenta
NF-Y 转录因子家族在适应环境应激过程中基因激活的功能分析。
  • 批准号:
    5441275
  • 财政年份:
    2004
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Durch photosensibilisierende Porphyrine induzierte Genexpression - Trägt die kombinatorische Vielfalt des Transkriptionsfaktors NF-Y zur stressinduzierten Antwort der Pflanze bei?
光敏卟啉诱导的基因表达 - 转录因子 NF-Y 的组合多样性是否有助于植物的胁迫诱导反应?
  • 批准号:
    5361724
  • 财政年份:
    2002
  • 资助金额:
    --
  • 项目类别:
    Research Units

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用于决策的前运动电路的分子和功能架构
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