Dissection of the tetrapyrrole-mediated signaling pathway: Identification of components involved in signal transduction and of target genes

剖析四吡咯介导的信号通路：鉴定参与信号转导的成分和靶基因

• 批准号: 42287343
• 负责人: Professor Dr. Bernhard Grimm
• 金额: --
• 依托单位: Arbeitsgruppe Pflanzenphysiologie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/42287343?language=en
• 关键词: Dissection; tetrapyrrole; mediated; signaling; pathway

Control of the essential tetrapyrrole biosynthesis requires retrograde signaling. Combinatorial analyses of global transcript profiles and identification of interaction partners of enzymes in the chlorophyll-synthesizing branch revealed that (I) chemically induced deregulation of expression of tetrapyrrole biosynthesis genes is a straight forward strategy to identify early up- and downregulated genes, (II) deregulated expression of enzymes rather than accumulation of specific Mg porphyrins affects nuclear gene expression, and (III) identified interaction partners likely contribute to the combination of different retrograde signals generated in plastids. The project aims to substantiate the mechanisms of tetrapyrrole pathway-mediated signaling. After evaluation of microarray and bioinformatics analyses target genes are identified and new components of the signaling pathway are proposed. Their contribution to retrograde signaling will be analyzed in transgenic lines by RNAi inactivation and overexpression. In parallel, a robust comparative analysis of the impact of tetrapyrrolic metabolites and effectors will be performed with photoautotrophic Arabidopsis cell cultures. Refined analyses of protein complexes for enzymes of the chlorophyll synthesis branch will be continued using transformants expressing tagged-proteins to reveal their contribution to control and signaling of the tetrapyrrole biosynthetic pathway. New insights are expected allowing a substantial assessment how tetrapyrrole metabolism is integrated in plastid-derived retrograde signaling.

控制必要的四吡咯生物合成需要逆行信号。对叶绿素合成分支中的全局转录谱和酶的相互作用配偶体的鉴定的组合分析揭示了（I）化学诱导的四吡咯生物合成基因的表达的失调是鉴定早期上调和下调基因的直接策略，（II）酶的表达失调而不是特定Mg卟啉的积累影响核基因表达，和（III）确定的相互作用伙伴可能有助于质体中产生的不同逆行信号的组合。该项目旨在证实四吡咯途径介导的信号转导机制。经过微阵列和生物信息学分析的评估后，确定了靶基因，并提出了信号通路的新组分。将通过RNAi失活和过表达在转基因系中分析它们对逆行信号传导的贡献。与此同时，将对光自养拟南芥细胞培养物进行四吡咯代谢物和效应物的影响的稳健比较分析。叶绿素合成分支的酶的蛋白质复合物的精细分析将继续使用表达标记蛋白质的转化体，以揭示它们对四吡咯生物合成途径的控制和信号传导的贡献。新的见解，预计允许大量的评估如何四吡咯代谢整合在质体衍生的逆行信号。