IDR: Clinical Ultrafast Laser Microsurgery Probe for Treatment of Scarred Vocal Folds

1014953 Ben-Yakar飞秒激光显微手术（FLMS）已成为一种卓越的技术，用于精确消融表面以下的活组织，同时确保对周围区域的损伤最小。该技术与非线性光学成像（NOI）模式（双光子荧光和二次谐波产生）的结合提供了一种非侵入性的可视化手段，以指导原位激光显微手术。一种能够结合FLMS和NOI的小型化探针将为医生提供一种具有最高精度的引导显微手术的方法。PI建议建立这样一个探头专门设计用于微妙的声带修复手术。他们将首先确定所需的激光参数，以便在有疤痕的声带中成功创建次表面空隙。其次，他们将建造和测试一种图像引导的微型手术探针。然后，他们将在活体动物瘢痕模型中进行手术，以确定这种新技术的有效性，然后再进入临床试验。

1014953Ben-YakarFemtosecond laser microsurgery (FLMS) has emerged as a remarkable technique for precisely ablating living tissue below the surface while ensuring minimal damage to surrounding regions. The combination of this technique with nonlinear optical imaging (NOI) modalities (two-photon fluorescence and second harmonic generation) provides a non-invasive means for visualization to guide laser microsurgeries in situ. A miniaturized probe capable of combined FLMS and NOI will provide physicians a means for guided microsurgery with the highest precision available. The PI proposes to build such a probe specifically designed for use in delicate vocal fold restoration surgeries. They will first determine the required laser parameters for successful creation of sub-surface voids in scarred vocal folds. Second they will build and test an image-guided, miniaturized surgery probe. They will then perform surgery in live animal scar models to determine the efficacy of this novel technique before it can move into clinical trials.