IDR: Clinical Ultrafast Laser Microsurgery Probe for Treatment of Scarred Vocal Folds

飞秒激光显微手术（FLMS）已成为一项卓越的技术，可以精确地消融表面以下的活组织，同时确保对周围区域的损害最小。该技术与非线性光学成像（NOI）模式（双光子荧光和二次谐波产生）的结合提供了一种非侵入性的可视化手段，以指导原位激光显微手术。一种能够结合FLMS和NOI的小型探针将为医生提供一种具有最高精度的引导显微手术的手段。PI建议建造这样一个探针，专门设计用于精细的声带修复手术。他们将首先确定所需的激光参数，以成功地在伤痕累累的声带中创建地下空洞。其次，他们将建造并测试一种图像引导的小型手术探头。然后，他们将在活体动物疤痕模型上进行手术，以确定这项新技术在进入临床试验之前的功效。

1014953Ben-YakarFemtosecond laser microsurgery (FLMS) has emerged as a remarkable technique for precisely ablating living tissue below the surface while ensuring minimal damage to surrounding regions. The combination of this technique with nonlinear optical imaging (NOI) modalities (two-photon fluorescence and second harmonic generation) provides a non-invasive means for visualization to guide laser microsurgeries in situ. A miniaturized probe capable of combined FLMS and NOI will provide physicians a means for guided microsurgery with the highest precision available. The PI proposes to build such a probe specifically designed for use in delicate vocal fold restoration surgeries. They will first determine the required laser parameters for successful creation of sub-surface voids in scarred vocal folds. Second they will build and test an image-guided, miniaturized surgery probe. They will then perform surgery in live animal scar models to determine the efficacy of this novel technique before it can move into clinical trials.