Chlamydia interacts with the Golgi apparatus - understanding the underlying molecular mechanisms
衣原体与高尔基体相互作用 - 了解潜在的分子机制
基本信息
- 批准号:197830553
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:德国
- 项目类别:Priority Programmes
- 财政年份:2011
- 资助国家:德国
- 起止时间:2010-12-31 至 2018-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Chlamydia spp. are obligate intracellular bacterial pathogens. Inside the eukaryotic host cell they are found in a membrane-bound compartment called the inclusion. Little is known about the molecular basis for specific interactions between this bacterial niche and cellular compartments, including the Golgi apparatus (GA). We have recently demonstrated that infection with C. trachomatis causes fragmentation of the GA, which is accompanied by processing of an important cellular Golgi matrix protein, golgin-84. Knockdown of the two GTPases, Rab6A and Rab11A, by RNA interference (RNAi) inhibited Chlamydia-induced and golgin-84-dependent Golgi fragmentation indicating that Rab proteins and golgin-84 are linked in a pathway that contributes to Golgi structure. Furthermore, our published data support the hypothesis that Golgi fragmentation is essential for bacterial lipid acquisition and Chlamydia growth. We will study trafficking of photoactivatable Rab6A, Rab11A and golgin-84 fusion proteins by confocal live cell microscopy to understand the dynamics of these proteins in infected and control cells. Characterization of Rab and golgin-84 interaction partners (known and novel) by biochemical means and RNAi will be used to elucidate the role of these factors in Chlamydia-induced Golgi fragmentation, bacterial lipid acquisition and development. The aim of the study is to understand the molecular basis of the processes by which Rab proteins and golgins cause Golgi fragmentation in infected cells to ensure Chlamydia lipid acquisition and growth.
衣原体属物种是专性细胞内细菌病原体。在真核宿主细胞内,它们存在于一个被称为内含物的膜结合区室中。关于这种细菌生态位和细胞区室(包括高尔基体(GA))之间特定相互作用的分子基础知之甚少。我们最近证明,感染C。沙眼衣原体引起GA的片段化,这伴随着重要的细胞高尔基体基质蛋白(golgin-84)的加工。通过RNA干扰(RNAi)敲低两种GTP酶Rab 6A和Rab 11 A抑制衣原体诱导的和高尔基体蛋白-84依赖的高尔基体片段化,表明Rab蛋白和高尔基体蛋白-84在有助于高尔基体结构的通路中连接。此外,我们发表的数据支持这一假设,高尔基体片段化是必不可少的细菌脂质收购和衣原体生长。我们将通过共聚焦活细胞显微镜研究光活化Rab 6A,Rab 11 A和golgin-84融合蛋白的运输,以了解这些蛋白在感染细胞和对照细胞中的动态。Rab和golgin-84相互作用的合作伙伴(已知的和新的)通过生化手段和RNAi的表征将被用来阐明这些因素在衣原体诱导的高尔基体片段化,细菌脂质收购和发展的作用。该研究的目的是了解Rab蛋白和golgins在感染细胞中引起高尔基体片段化以确保衣原体脂质获得和生长的过程的分子基础。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Dr. Dagmar Heuer其他文献
Dr. Dagmar Heuer的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Dr. Dagmar Heuer', 18)}}的其他基金
Deciphering the role of spingolipids in bacterial egress
破译鞘脂在细菌排出中的作用
- 批准号:
446458511 - 财政年份:
- 资助金额:
-- - 项目类别:
Priority Programmes
相似海外基金
Elucidation of the molecular mechanisms by which pectin interacts with small intestinal cells
阐明果胶与小肠细胞相互作用的分子机制
- 批准号:
22H02285 - 财政年份:2022
- 资助金额:
-- - 项目类别:
Grant-in-Aid for Scientific Research (B)
Wind, Water & Ice: How Erosion Interacts with Climate and Tectonics to Shape the Landscape
风、水
- 批准号:
RGPIN-2020-05956 - 财政年份:2022
- 资助金额:
-- - 项目类别:
Discovery Grants Program - Individual
Next-generation humanized mice to investigate how Traumatic Brain Injury interacts with genetic risk factors to trigger dementia
下一代人源化小鼠研究创伤性脑损伤如何与遗传风险因素相互作用以引发痴呆
- 批准号:
486349 - 财政年份:2022
- 资助金额:
-- - 项目类别:
Studentship Programs
Improved Representation of Cloud-Aerosol Interactions in the Community Earth System Model: A New Sectional Cloud Model that Interacts with Modal and Sectional Aerosol Models
社区地球系统模型中云-气溶胶相互作用的改进表示:与模态和剖面气溶胶模型相互作用的新剖面云模型
- 批准号:
2114638 - 财政年份:2021
- 资助金额:
-- - 项目类别:
Standard Grant
Wind, Water & Ice: How Erosion Interacts with Climate and Tectonics to Shape the Landscape
风、水
- 批准号:
RGPIN-2020-05956 - 财政年份:2021
- 资助金额:
-- - 项目类别:
Discovery Grants Program - Individual
Meteorological Islands: How the Atmosphere Interacts with Large Individual Patches of Heterogeneity
气象岛屿:大气如何与大的异质性个体斑块相互作用
- 批准号:
2128345 - 财政年份:2021
- 资助金额:
-- - 项目类别:
Standard Grant
HIF1-alpha interacts with MIF to drive inflammation and new bone formation in spondyloarthritis
HIF1-α 与 MIF 相互作用,促进脊柱关节炎中的炎症和新骨形成
- 批准号:
445586 - 财政年份:2021
- 资助金额:
-- - 项目类别:
Operating Grants
Realization of a Life Support Robot that Plays and Interacts with Children through Dynamic Physical Contact
通过动态身体接触与儿童玩耍和互动的生命支持机器人的实现
- 批准号:
20K04389 - 财政年份:2020
- 资助金额:
-- - 项目类别:
Grant-in-Aid for Scientific Research (C)
Investigating how sleep interacts with neuroligin to mediate synaptic plasticity underlying long-term memory using Caenorhabditis elegans
使用秀丽隐杆线虫研究睡眠如何与 Neuroligin 相互作用来介导长期记忆的突触可塑性
- 批准号:
553366-2020 - 财政年份:2020
- 资助金额:
-- - 项目类别:
Alexander Graham Bell Canada Graduate Scholarships - Master's
Wind, Water & Ice: How Erosion Interacts with Climate and Tectonics to Shape the Landscape
风、水
- 批准号:
RGPIN-2020-05956 - 财政年份:2020
- 资助金额:
-- - 项目类别:
Discovery Grants Program - Individual