权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Biogenesis of novel membrane compartments in Salmonella enterica-infected host cells

肠道沙门氏菌感染宿主细胞中新型膜区室的生物发生

基本信息

批准号：
198065218
负责人：
Professor Dr. Michael Hensel
金额：
--
依托单位：
Arbeitsgruppe Mikrobiologie
依托单位国家：
德国
项目类别：
Priority Programmes
财政年份：
2011
资助国家：
德国
起止时间：
2010-12-31 至 2018-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/198065218?language=en
关键词：
Biogenesis novel membrane compartments Salmonella

项目摘要

Salmonella enterica is a facultative intracellular pathogen that inhabits a unique membrane-bound compartment, termed Salmonella-containing vacuole (SCV). Intracellular Salmonella induce the massive remodeling of the host endosomal system, resulting in tubular membrane aggregated such as Salmonella-induced filaments and further tubular structures. Based on our novel observations on ultrastructural and dynamic properties of host membranes modified by Salmonella, the project will explore the cellular and molecular basis of SCV and tubular compartment formation. These analyses will deploy electron microscopy (EM) and immuno-EM. Host membranes modified by activities of intracellular Salmonella will be specifically enriched and subjected to analyses of the proteome and lipidome. These analyses should complement the investigation of cellular origin of the unique com-partments. For a detailed understanding of the composition of the SCV, specific reporter assays will be developed to study the interaction of bacteria within the SCV with the content of other compartments of the host cell. Due to the heterogeneity of the intracellular bacteria, these analyses will be directed to individual bacteria serving as reporters. The role of Salmonella-derived membrane material in the biogenesis of novel membrane compartments will be analysed.

肠沙门氏菌是一种兼性细胞内病原体，栖息在一个独特的膜结合区室中，称为含沙门氏菌液泡 (SCV)。细胞内沙门氏菌诱导宿主内体系统的大规模重塑，导致管状膜聚集，例如沙门氏菌诱导的细丝和进一步的管状结构。基于我们对沙门氏菌修饰的宿主膜的超微结构和动态特性的新观察，该项目将探索 SCV 和管状室形成的细胞和分子基础。这些分析将采用电子显微镜 (EM) 和免疫-EM。通过细胞内沙门氏菌活性修饰的宿主膜将被专门富集并进行蛋白质组和脂质组分析。这些分析应该补充独特隔室的细胞起源的研究。为了详细了解 SCV 的组成，将开发特定的报告测定法来研究 SCV 内细菌与宿主细胞其他区室内容物的相互作用。由于细胞内细菌的异质性，这些分析将针对充当报告基因的单个细菌。将分析沙门氏菌衍生的膜材料在新型膜区室的生物发生中的作用。