The structural and functional basis of reprogramming of vesicular transport by Salmonella enterica effector proteins

肠道沙门氏菌效应蛋白重编程囊泡运输的结构和功能基础

• 批准号: 186419184
• 负责人: Professor Dr. Michael Hensel
• 金额: --
• 依托单位: Arbeitsgruppe Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/186419184?language=en
• 关键词: structural; functional; basis; reprogramming; vesicular

Salmonella enterica is a facultative intracellular pathogen that resides and replicates within a membrane-bound compartment of host cells, the so-called ‘Salmonella-containing vacuole’ or SCV. Intracellular Salmonella deploy a type III secretion system (T3SS) encoded by Salmonella Pathogenicity Island 2 (SPI2) to manipulate host cell functions and to achieve intracellular proliferation. The project should lead to the understanding of molecular basis of these manipulations. We aim to i) define the role of individual SPI2-T3SS effectors in modification of cellular transport and remodeling of the endosomal system, ii) characterize host factors involved in remodeling of the endosomal system by Salmonella, iii) identify interaction partners of effectors by genetic screens and protein interaction analyses, and ultimately iv) to determine the structure/function relationship of SPI2-T3SS effectors. The identification and analyses of effector/target interactions in the proposed project will lead to a molecular understanding of the intracellular strategies of an important intracellular pathogen.

肠道沙门氏菌是一种兼性细胞内病原体，其在宿主细胞的膜结合区室（所谓的“含沙门氏菌的空泡”或SCV）内驻留和复制。沙门氏菌胞内部署由沙门氏菌致病岛2（SPI 2）编码的III型分泌系统（T3 SS）来操纵宿主细胞功能并实现胞内增殖。该项目应导致这些操作的分子基础的理解。我们的目标是i）定义单个SPI 2-T3 SS效应物在细胞转运的修饰和内体系统的重塑中的作用，ii）表征沙门氏菌内体系统重塑中涉及的宿主因子，iii）通过遗传筛选和蛋白质相互作用分析鉴定效应物的相互作用伴侣，以及最终iv）确定SPI 2-T3 SS效应物的结构/功能关系。在拟议的项目中的效应/靶相互作用的识别和分析将导致一个重要的细胞内病原体的细胞内策略的分子理解。

项目成果

SseF, a type III effector protein from the mammalian pathogen Salmonella enterica, requires resistance-gene-mediated signalling to activate cell death in the model plant Nicotiana benthamiana.

• DOI: 10.1111/j.1469-8137.2012.04124.x
• 发表时间: 2012-06
• 期刊: The New phytologist
• 作者: Suayib Ustün;P. Müller;R. Palmisano;M. Hensel;F. Börnke

Live Cell Imaging Reveals Novel Functions of Salmonella enterica SPI2-T3SS Effector Proteins in Remodeling of the Host Cell Endosomal System

活细胞成像揭示肠道沙门氏菌 SPI2-T3SS 效应蛋白在宿主细胞内体系统重塑中的新功能

• DOI: 10.1371/journal.pone.0115423
• 发表时间: 2014
• 期刊: PLoS ONE
• 影响因子: 3.7
• 作者: Rajashekar;Chikkaballi;Hensel
• 通讯作者: Hensel