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Arabidopsis 2010: Transcription and Processing Requirements for Noncoding Transcripts Near SiRNA Loci in Arabidopsis

拟南芥 2010：拟南芥中 SiRNA 位点附近非编码转录本的转录和处理要求

基本信息

批准号：
1021465
负责人：
Xuemei Chen
金额：
$ 74.7万
依托单位：
University of California-Riverside
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2010
资助国家：
美国
起止时间：
2010-09-01 至 2013-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1021465&HistoricalAwards=false
关键词：
Arabidopsis 2010 Transcription Processing Requirements

项目摘要

In the past decade, genome-wide profiling of transcripts in plants, animals, and fungi have revealed pervasive transcription in the genome and the presence of multitudes of noncoding transcripts, which do not encode protein products. Increasing evidence points to an essential role of nuclear noncoding RNAs in the regulation of gene expression or genome stability. However, the mechanisms underlying the transcription, processing, and degradation of nuclear noncoding transcripts are almost completely unknown. This project aims to systematically identify nuclear noncoding RNAs by high-throughput sequencing, and to study the mechanisms governing the biogenesis and processing of a subset of such noncoding transcripts, the ones that participate in transcriptional gene silencing to ensure genome stability. The project is part of a long-term effort towards the full understanding of the roles of RNAs in the regulation of genes and genomes. Broader impacts: This project will illuminate the landscape and biogenesis requirements of plant noncoding RNAs, and thus set the foundation for the scientific community to dissect previously under-appreciated mechanisms of gene and genome regulation. The project will bridge disciplines through collaboration between biologists and computer scientists, train postdoctoral fellows and graduate students in biology to become well versed in bioinformatics, and provide research opportunities in biology to undergraduate students including computer science students.

在过去的十年中，植物，动物和真菌的全基因组转录谱揭示了基因组中普遍存在的转录和大量的非编码转录本，它们不编码蛋白质产物。越来越多的证据表明，核非编码RNA在基因表达或基因组稳定性的调节中起着重要作用。然而，核非编码转录本的转录、加工和降解机制几乎完全未知。该项目旨在通过高通量测序系统地鉴定核非编码RNA，并研究这些非编码转录本的生物发生和加工机制，这些转录本参与转录基因沉默以确保基因组稳定性。该项目是长期努力的一部分，旨在充分了解RNA在基因和基因组调控中的作用。更广泛的影响：该项目将阐明植物非编码RNA的景观和生物发生要求，从而为科学界解剖以前未被充分认识的基因和基因组调控机制奠定基础。该项目将通过生物学家和计算机科学家之间的合作来连接学科，培养生物学博士后研究员和研究生精通生物信息学，并为包括计算机科学学生在内的本科生提供生物学研究机会。