Development of Instrumentation for High Throughput, Single Nanoparticle Tracking and LSPR Biosensing

化学系的化学测量和成像计划（CMI）支持西北大学的Richard P.货车Duyne教授及其团队开发快速并行生物传感的新仪器和概念。 长期的目标是使许多新的实验，如（1）实时三维纳米粒子跟踪在低粘度介质中研究纳米粒子的生长动力学;（2）扩展生物传感器的灵敏度到单分子的限制;（3）开发利用单一纳米粒子的多路生物传感器;（4）测量与分子连接器构建的纳米粒子组装体的内部波动动力学;（5）测量纳米粒子组装体的内部波动动力学。（5）细胞内生物传感。 开发的仪器将导致从根本上对吸附到单个纳米颗粒上的单分子（或小簇）的化学性质的新理解。这项研究有可能对以下方面产生广泛的影响：（1）一般的纳米科学技术，特别是等离子体;（2）研究生，博士后学者，本科生和中学教师的教育;（3）为工业和学术界的职业培训妇女和少数民族科学家;（4）向广大受众传播有关纳米科学技术的信息。大量的科学和社会影响将来自于应用，如新的等离子体传感器在体内葡萄糖监测，临床诊断阿尔茨海默氏症？的疾病，化学/生物战剂检测和艺术保护研究。从事该项目的学生和博士后将接受机械和电子结构以及仪器控制编程方面的广泛培训。 因此，他们将拥有成为化学测量科学独立和创新研究领导者所需的工具集。

The Chemical Measurement and Imaging program (CMI) of the Division of Chemistry supports Professor Richard P. Van Duyne and his group at Northwestern University to develop new instrumentation and concepts for fast, parallel biosensing. The long-term aim is to enable many new experiments such as (1) real-time 3D nanoparticle tracking in low viscosity media to study nanoparticle growth dynamics; (2) extending the biosensor sensitivity to the single molecule limit; (3) developing multiplexed biosensors utilizing single nanoparticles; (4) measuring the internal fluctuational dynamics of nanoparticle assemblies constructed with molecular linkers; and (5) intracellular biosensing. The instrumentation developed will lead to fundamentally new understanding of the chemistry of single molecules (or small clusters) adsorbed onto single nanoparticles. This research has potential for broad impact on: (1) nanoscale science & technology in general and plasmonics in particular; (2) the education of graduate students, postdoctoral scholars, undergraduates, and secondary school teachers; (3) the training of women and minority scientists for careers in industry and academia; and (4) the dissemination of information about nanoscale science & technology to broad audiences. Substantial scientific and societal impact will derive from applications such as new plasmonic sensors for in vivo glucose monitoring, clinical diagnostics for Alzheimer?s disease, chemical/biological warfare agent detection, and art conservation studies. Students and postdocs working on this project will receive extensive training in mechanical and electronics construction as well as instrument control programming. As a consequence they will have the tool set needed to become independent and innovative research leaders in chemical measurement science.