IDBR: High-Throughput Instrumentation for Lipid Bilayers and Patch-clamp

IDBR：脂质双层和膜片钳的高通量仪器

• 批准号: 1154498
• 负责人: Eugenio Culurciello
• 金额: $ 66.8万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-15 至 2014-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1154498&HistoricalAwards=false
• 关键词: IDBR; Throughput; Instrumentation; Lipid; Bilayers

Award Abstract:Proposal Number: 1063347 Proposal Title: IDBR: High throughput instrumentation for lipid bilayers and single-channel patch-clamp This award supports a project that will develop integrated micro-chip instrumentation used for recording the minute currents across biological cell membranes. The goal is an improvement of the sensitivity and the throughput of electrophysiology instruments for the study of membrane proteins, including functional analysis of ion channels and DNA identification using special channels with nano-scale pores. This project will also advance the sensitivity of patch-clamp recording systems and will allow inspection of the opening and closing of individual ion-channels whose conductance has previously been too low to examine. The proposed instrument will increase the number of recording channels by several hundred times. The research will advance the design of ultra-low noise instruments that maintain a small footprint and can be manufactured with standard and cheap microchip technologies. We will design, instrument and test integrated circuitry to increase the density of recording sites and miniaturize the recording equipment. Current measurements are used throughout biomedical instruments and thus the work proposed here will have large applicability in medicine and engineering. The amplifier we will develop can be used in instruments for cellular biology and physiology, DNA sequencing with nanopores, low-power circuit for cellular interfaces, nano-sensors and biomedical nano- devices, low-noise acquisition of bio-signals. The societal impacts of the technology and ion-channel research are in the prevention of diseases, and in many other scientific fields that rely on cheap recording of genetic material, such as forensics, biology, anthropology. Low-cost high-throughput electrophysiology devices also improve drug development and delivery, both for disease prevention, gene therapy, as well as for personalized medicine. The principal investigators are involved in the Science Saturdays program for under-represented minority and women students. Within this acclaimed program, the PIs are working with children and teachers from K to 12, to promote practical science projects and to develop interest, abilities and communication skills. The investigators excite students interest with presentations in local schools, participation to the New Haven Science Fair, organizing laboratory tours and weekend science projects.

获奖摘要：提案编号：1063347提案标题：IDBR：脂质双层和单通道膜片钳的高通量仪器该奖项支持一个项目，该项目将开发用于记录跨生物细胞膜的微小电流的集成微芯片仪器。目标是提高电生理仪器的灵敏度和通量，用于研究膜蛋白，包括离子通道的功能分析和使用具有纳米级孔的特殊通道的DNA鉴定。该项目还将提高膜片钳记录系统的灵敏度，并将允许检查电导率以前太低而无法检查的单个离子通道的打开和关闭。拟议的仪器将使记录通道的数量增加数百倍。这项研究将推进超低噪声仪器的设计，这些仪器保持小的占地面积，可以用标准和廉价的微芯片技术制造。我们将设计，仪器和测试集成电路，以增加记录点的密度和记录设备的集成化。电流测量在整个生物医学仪器中使用，因此这里提出的工作在医学和工程中具有很大的适用性。我们将开发的放大器可用于细胞生物学和生理学仪器，纳米孔DNA测序，细胞接口的低功耗电路，纳米传感器和生物医学纳米器件，生物信号的低噪声采集。这项技术和离子通道研究的社会影响是在预防疾病方面，以及在许多其他依赖于廉价记录遗传物质的科学领域，如法医学，生物学，人类学。低成本高通量电生理设备还可以改善药物开发和输送，用于疾病预防、基因治疗以及个性化医疗。主要研究人员参与了为代表性不足的少数民族和女学生举办的科学星期六项目。在这个广受好评的计划中，PI与K到12岁的儿童和教师合作，促进实用科学项目，培养兴趣，能力和沟通技巧。研究人员通过在当地学校的演讲、参加纽黑文科学博览会、组织实验室图尔斯参观和周末科学项目来激发学生的兴趣。