Effects of Elasticity and Geometry on Cellular Uptake of Nanoparticles

弹性和几何形状对纳米颗粒细胞摄取的影响

• 批准号: 1028530
• 负责人: Huajian Gao
• 金额: $ 38.09万
• 依托单位: Brown University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1028530&HistoricalAwards=false
• 关键词: Effects; Elasticity; Geometry; Cellular; Uptake

Effects of elasticity and geometry on cellular uptake of nanoparticlesPI: Huajian Gao, Brown UniversityAbstractThe objective of this proposal is to investigate the effects of elasticity and geometry on cellular uptake of nanoparticles based on a number of theoretical and simulation techniques developed by the PI?s research group on endocytosis and cell mechanics. The proposed work will include development of theoretical models of receptor-mediated endocytosis, calculations of free energy and phase diagrams of cell-particle attachment, interaction between elastic particles and cell membrane, cellular uptake of particles with large aspect ratios, as well as ultra-large scale coarse-grained molecular dynamics simulations of carbon nanotubes docking and wrapping into cell membranes via non-specific and specific interaction forces.Intellectual Merit:The proposed project complements existing multidisciplinary experimental research programs at Brown University on nanotoxicology in addressing the current lack of understanding on the roles of elasticity and aspect ratio of nanoparticles in the cellular uptake of carbon nanotubes and other potentially toxic nanoparticles. The proposed research will contribute to the fundamental understanding of mechanisms by which nanomaterials enter human and animal cells, an issue of tremendous societal concern regarding both beneficial and potential hazardous effects of nanotechnology which are projected to produce and release thousands of tons of nanomaterials into the environment in the coming decades. The project can also provide insights or guidelines for the development of efficient gene and drug targeting and delivery systems in biomedical technology. Broad impact:The proposed work at the interface between a number of traditional academic disciplines including mechanics, biology, biotechnology, nanotechnology, materials science, chemistry and physics will generate new knowledge and fundamental understanding that may provide insights and guidelines for a wide range of applications in nano- and bio-technologies. The educational components of the proposed research include training of a PhD student, development of two graduate courses in biomechanics, research experience and mentoring of students from under-represented groups and participation in the outreach programs to historically black colleges and K-12 in the Brown MRSEC program.

弹性和几何形状对细胞摄取的nanoparticlesPI的影响：高华健，布朗大学AbstractThe本提案的目的是研究弹性和几何形状对细胞摄取的纳米粒子的基础上，一些理论和模拟技术开发的PI？内吞作用和细胞力学的研究小组。拟议的工作将包括发展受体介导的内吞作用的理论模型，计算细胞-颗粒附着的自由能和相图，弹性颗粒与细胞膜之间的相互作用，细胞对大纵横比颗粒的摄取，以及碳纳米管对接和包裹到细胞膜通过非-智力优点：拟议的项目补充现有的多学科实验研究计划在布朗大学纳米毒理学在解决目前缺乏了解的弹性和纵横比的纳米粒子在碳纳米管和其他潜在的有毒纳米粒子的细胞摄取的作用。拟议的研究将有助于从根本上了解纳米材料进入人类和动物细胞的机制，这是一个关于纳米技术的有益和潜在危险影响的巨大社会问题，预计在未来几十年内将产生数千吨纳米材料并将其释放到环境中。该项目还可以为生物医学技术中有效的基因和药物靶向和递送系统的开发提供见解或指导方针。广泛的影响：在一些传统的学术学科，包括力学，生物学，生物技术，纳米技术，材料科学，化学和物理学之间的接口拟议的工作将产生新的知识和基本的理解，可能提供的见解和指导方针，在纳米和生物技术的广泛应用。拟议研究的教育组成部分包括博士生的培训，生物力学两个研究生课程的开发，研究经验和来自代表性不足群体的学生的指导，以及参与外展计划，以历史上的黑人大学和布朗MRSEC计划中的K-12。