Collaborative Research: Changing the manganese paradigm

该项目是特拉华州大学和俄勒冈州健康与科学大学的研究人员之间的合作，将开发一种灵敏的方法，用于测量海洋沃茨中亚纳摩尔水平的总锰和锰（III）的浓度，无论是在氧化环境中还是在还原区。所提出的方法是一个修改的已发表的方法，它具有nM的灵敏度。该方法的检测限的改进和其测量环境水平（0.5 nM）的Mn（III）的能力的证明是这个为期一年的项目的主要目标。两种不同的方法将进行调查，初步证据表明，这两种方法都是可行的替代方案。使用长光程光学池将增加当前检测系统的灵敏度，并且预浓缩是可以使用的另一种方法。另一种方法是使用荧光检测，这也将进行研究。将利用观测资料，包括最近海洋学相互校准活动中收集的资料，对各种方法进行测试。此外，这些方法将在短期航行和实验室培养实验中进行测试。更广泛的影响：锰的氧化还原化学会影响许多全球生物化学循环，因此了解这些相互作用具有广泛的意义。该项目将培训研究生，并将通过一系列教育和公共宣传活动宣传其成果。这项研究将继续在以前资助的工作中开展国际合作。

The project, a collaboration between investigators at the University of Delaware and Oregon Health and Science University, will develop a sensitive method for measuring the concentration of total Mn and Mn(III) at sub-nanomolar levels in ocean waters, both in oxic environments and in reduced zones. The proposed method is a modification of a published method, which has nM sensitivity. The improvement in the detection limit of this method and the demonstration of its ability to measure Mn(III) at environmental levels (0.5 nM)is the major goal of this one year project. Two different approaches will be investigated, and preliminary evidence suggests that both approaches are viable alternatives. Use of a long path optical cell will increase sensitivity of the current detection system, and preconcentration is another approach that can be used. An alternative is the use of fluorescence detection, and this will also be investigated. The various methods will be tested using referrence materials, including those collected during recent oceanographic intercalibration exercises. Additionally, the methods will be tested during short cruises and through laboratory culture experiments.Broader Impacts: Manganese redox chemistry impacts a number of global biogeochmeical cycles, and therefore understanding these interactions has broad implications. The project will train graduate students, and the results will be desseminated through a number of educational and public outreach activities. This research will continue international collaborations developed during previously funded work.