Collaborative Research: How bacteria control manganese(IV) oxide biomineralization using a multicopper oxidase complex

合作研究：细菌如何使用多铜氧化酶复合物控制氧化锰（IV）生物矿化

• 批准号: 2120408
• 负责人: Bradley Tebo
• 金额: $ 42万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-01-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2120408&HistoricalAwards=false
• 关键词: Collaborative; Research; How; bacteria; control

With this award, the Chemistry of Life Processes Program in the Chemistry Division is funding Dr. Bradley Tebo from Oregon Health & Science University and Dr. Thomas Spiro from the University of Washington to find out how bacteria change dissolved manganese (Mn) into manganese oxide minerals. This chemical process produces manganese oxides that are widespread, highly reactive, efficient catalysts that play important roles in elemental biogeochemical cycles. Although these oxides are primarily made by microbial processes, the biological means for their formation are poorly understood. This project reveals the pathway of bacterial manganese oxide production, using the first purified active manganese-oxidizing enzyme complex. The project may uncover the biochemistry of how two processes-manganese oxidation and mineral formation-are achieved, laying the foundation for future application of biologically-derived manganese oxides in clean water technologies and energy storage and production. This project enhances the training of the next generation of scientists in interdisciplinary methods, and serves as a vehicle in outreach programs to engage undergraduate, middle, and high school students in studies of microbiology and biochemistry, as related to environmental science. In addition, postdoctoral fellows develop a Saturday Academy course targeting either 6-8 or 9-12 grade students and focusing on the importance of microbiology and biochemistry as related to environmental science. The Saturday Academy provides professional development for the postdoctoral fellow as the instructor.The project represents an investigation of how a purified protein can accomplish the difficult two-electron oxidation of Mn(II), forming a nanoparticulate MnO2 mineral product. Results to date indicate that the active protein belongs to the well-known multicopper oxidase family of enzymes, but with a unique ability to adapt the polynuclear chemistry of manganese to perform the catalysis. The research efforts are focused on establishing a molecular-level detailed mechanism of the catalysis, by resolving manganese oxidation intermediates and studying the effects of key mutations with an array of spectroscopic techniques. The spectroscopic studies are complemented by modeling, crystallography, and cryo-electron microscopy work to derive the 3D atomic structure of the enzyme. In the context of broader environmental applicability, the research explores the effect of other metals occurring in the environment on the enzymatic catalysis of manganese oxidation.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

有了这个奖项，化学部的生命过程化学计划正在资助俄勒冈州健康科学大学的布拉德利特博博士和华盛顿大学的托马斯斯皮罗博士，以了解细菌如何将溶解的锰（Mn）转化为氧化锰矿物。这种化学过程产生的锰氧化物是广泛存在的、高活性的、有效的催化剂，在元素地球化学循环中发挥重要作用。虽然这些氧化物主要是由微生物过程，其形成的生物手段知之甚少。该项目揭示了细菌氧化锰生产的途径，使用第一个纯化的活性锰氧化酶复合物。该项目可能揭示锰氧化和矿物形成两个过程的生物化学，为生物衍生的锰氧化物在清洁水技术和能源储存和生产中的未来应用奠定基础。该项目加强了跨学科方法下一代科学家的培训，并作为外展计划的工具，让本科生，初中生和高中生参与与环境科学相关的微生物学和生物化学研究。 此外，博士后研究员开发了一个周六学院课程，针对6-8或9-12年级的学生，重点是与环境科学相关的微生物学和生物化学的重要性。星期六学院为博士后研究员提供专业发展作为指导。该项目代表了一种纯化的蛋白质如何完成Mn（II）的困难的双电子氧化，形成纳米颗粒MnO 2矿物产品的研究。迄今为止的结果表明，活性蛋白属于众所周知的多铜氧化酶家族的酶，但具有独特的能力，以适应锰的多核化学进行催化。研究工作的重点是通过解析锰氧化中间体并利用一系列光谱技术研究关键突变的影响，建立分子水平的详细催化机制。光谱研究通过建模，晶体学和冷冻电子显微镜工作来补充，以获得酶的3D原子结构。在更广泛的环境适用性的背景下，该研究探索了环境中其他金属对锰氧化酶催化的影响。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Metallo-inhibition of Mnx, a bacterial manganese multicopper oxidase complex

Mnx（一种细菌锰多铜氧化酶复合物）的金属抑制

• DOI: 10.1016/j.jinorgbio.2021.111547
• 发表时间: 2021
• 期刊: Journal of Inorganic Biochemistry
• 影响因子: 3.9
• 作者: Soldatova, Alexandra V.;Fu, Wen;Romano, Christine A.;Tao, Lizhi;Casey, William H.;Britt, R. David;Tebo, Bradley M.;Spiro, Thomas G.
• 通讯作者: Spiro, Thomas G.