Nanofiber-Based Sensors for Oxygen Determination in Model Glioblastomas

1033991 Palmer拟议的研究将开发一种基于纳米纤维的传感器，能够量化迁移性胶质母细胞瘤群体内部和周围的氧分布。PI已经表明，静电纺丝排列的纤维阵列可以引导粘附的人源性肿瘤细胞的迁移。通过制造“核-壳”纤维，可以将氧敏感指示剂添加到该纤维的“核”中，而“壳”保护细胞免于暴露于指示剂。共聚焦显微镜可以询问氧含量，并使实验涉及一个或多个神经球，它们如何与周围的[O2]和彼此相互作用。 在人体内重建低氧、常氧和高氧条件，同时鼓励已知为肿瘤转移组成部分的迁移的传感器系统将可用于许多人类癌症的研究。此外，传感能力将导致对在器官生成和替换期间新的正常人体组织的创建和整合中出现的类似问题的更多理解。

1033991PalmerThe proposed research will develop a nanofiber-based sensor capable of quantifying oxygen distributions within and around populations of migrating glioblastoma populations. The PI has already shown that electrospun aligned fiber arrays can guide the migration of adherent human-derived tumor cells. By fabricating a "core-shell" nanofiber, oxygen-sensitive indicators can be added to the "core" of this fiber while the "shell" protects cells from exposure to the indicator. Confocal microscopy can interrogate oxygen content and enable experiments involving one or more neurospheres, how they interact with the surrounding [O2] and with each other. A sensor system that recreates hypoxic, normoxic, and hyperoxic conditions within the human body while simultaneously encouraging migration known to be a component of tumor metastasis will be useful in the study of many human cancers. Furthermore, the sensing capability will lead to increased understanding of similar issues that arise in the creation and integration of new, normal human tissue during organ generation and replacement.