MRI: Acquisition of a Fluorescence Activated Cell Sorter for Research and Training at Ohio University

1039869 goetz该提案是购买最先进的具有细胞分选能力的流式细胞仪，特别是由BD Biosciences生产的FACSAria特别订单（SO）。FACSAria SO将支持来自俄亥俄大学校园3个学院的研究人员，代表6个系和众多本科和研究生学术项目，包括2个跨学科研究生项目。主要用户有研究生24人，本科生14人，高级人员6人。大多数主要用户通过与校园范围内的生物工程/生物技术倡议的正式联系而统一。这些教员之间的合作涉及生物工程、生物物理学、化学和细胞生物学。这些研究项目是非常有价值的，从教师的出版记录和同行评审的研究支持中可以看出。FACSAria SO将取代俄亥俄大学现有的流式细胞仪。目前的流式细胞仪是16年前从BD Pharmingen（现为BD Biosciences）购买的，不再生产。目前的流式细胞仪的技术过时和不可升级的能力严重限制了研究进展和培训。此外，仪器的频繁故障导致不可接受的挫败感和生产力损失。审稿人评论了俄亥俄大学缺乏足够的流式细胞仪设备。最近的全功能流式细胞仪/细胞分选仪位于俄亥俄州哥伦布市，距离俄亥俄大学75英里。

1039869GoetzThis proposal is for the purchase of a state-of-the-art flow cytometer with cell sorting capability, specifically a FACSAria Special Order (SO) manufactured by BD Biosciences. The FACSAria SO will support investigators from 3 colleges on the Ohio University campus representing 6 departments and a multitude of undergraduate and graduate academic programs including 2 interdisciplinary graduate programs. Combined the major users have 24 graduate students, 14 undergraduate students, and 6 senior personnel. The majority of the primary users are unified through formal affiliation with campus-wide bioengineering/biotechnology initiatives. The numerous ongoing collaborations among these faculty members span bioengineering, biophysics, chemistry, and cell biology. The research projects are exceedingly meritorious as evidenced by the faculty members' publication records and the peer-reviewed research support. The FACSAria SO will replace the existing flow cytometer owned by Ohio University. The current flow cytometer was purchased more than 16 years ago from BD Pharmingen (currently BD Biosciences) and is no longer manufactured. The outdated technology and non-upgradeable capabilities of the current flow cytometer are severely limiting research progress and training. In addition, the frequent breakdown of the instrument leads to an unacceptable amount of frustration and lost productivity. Reviewers have commented on the lack of adequate flow cytometry facilities at Ohio University. The nearest fully functional flow cytometer/cell sorter is located in Columbus, Ohio, 75 miles away from Ohio University.