MRI: Acquisition of a Fluorescence Activated Cell Sorter for Research and Training at Ohio University

1039869 Goetz本提案旨在购买具有细胞分选能力的最先进流式细胞仪，特别是BD Biosciences生产的FACSAria Special Order（SO）。FACSAria SO将支持来自俄亥俄州大学校园3所学院的调查人员，代表6个部门和众多的本科和研究生学术课程，包括2个跨学科的研究生课程。合并后的主要用户有24名研究生，14名本科生，6名高级人员。大多数主要用户通过与校园范围内的生物工程/生物技术倡议的正式联系而统一起来。这些教师之间的许多正在进行的合作跨越生物工程，生物物理学，化学和细胞生物学。这些研究项目是非常值得称赞的，这一点可以从教师的出版记录和同行评审的研究支持中得到证明。FACSAria SO将取代俄亥俄州大学现有的流式细胞仪。目前的流式细胞仪是16年前从BD Pharmingen（目前为BD Biosciences）购买的，已不再生产。目前流式细胞仪的过时技术和不可拆卸的能力严重限制了研究进展和培训。此外，仪器的频繁故障导致了不可接受的挫折和生产力损失。评审员对俄亥俄州大学缺乏足够的流式细胞仪设施发表了评论。最近的全功能流式细胞仪/细胞分选仪位于俄亥俄州的哥伦布，距离俄亥俄州大学75英里。

1039869GoetzThis proposal is for the purchase of a state-of-the-art flow cytometer with cell sorting capability, specifically a FACSAria Special Order (SO) manufactured by BD Biosciences. The FACSAria SO will support investigators from 3 colleges on the Ohio University campus representing 6 departments and a multitude of undergraduate and graduate academic programs including 2 interdisciplinary graduate programs. Combined the major users have 24 graduate students, 14 undergraduate students, and 6 senior personnel. The majority of the primary users are unified through formal affiliation with campus-wide bioengineering/biotechnology initiatives. The numerous ongoing collaborations among these faculty members span bioengineering, biophysics, chemistry, and cell biology. The research projects are exceedingly meritorious as evidenced by the faculty members' publication records and the peer-reviewed research support. The FACSAria SO will replace the existing flow cytometer owned by Ohio University. The current flow cytometer was purchased more than 16 years ago from BD Pharmingen (currently BD Biosciences) and is no longer manufactured. The outdated technology and non-upgradeable capabilities of the current flow cytometer are severely limiting research progress and training. In addition, the frequent breakdown of the instrument leads to an unacceptable amount of frustration and lost productivity. Reviewers have commented on the lack of adequate flow cytometry facilities at Ohio University. The nearest fully functional flow cytometer/cell sorter is located in Columbus, Ohio, 75 miles away from Ohio University.