MRI: Acquisition of a fluorescence activated cell sorter (FACS) supporting multi-disciplinary approaches to exploring biological responses

MRI：获取荧光激活细胞分选仪 (FACS)，支持多学科方法探索生物反应

• 批准号: 2117146
• 负责人: Mary Miller
• 金额: $ 35.77万
• 依托单位: Rhodes College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-10-01 至 2024-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2117146&HistoricalAwards=false
• 关键词: MRI; Acquisition; fluorescence; activated; cell

An award is made to Rhodes College to support the acquisition of a fluorescence activated cell sorter (FACS) to enhance the research of faculty and training of undergraduate students at Rhodes College and institutions in the Memphis area. This instrument allows separation of small particles, including living cells, from large populations and is based on very specific features such as particle size or the presence of a protein. As cells are isolated, very detailed information can be gathered about each cell and the living cells can be further studied. Addition of the FACS instrument will transform the ability to train the next generation of scientists in Rhodes faculty-led coursework, mentored research, and outreach programs at this research-active Primarily Undergraduate Institution (PUI). This instrument allows fast and precise analysis and separation that is very robust so that it will help scientists and undergraduate students answer a broad range of biological questions, including the how genes function, how cells respond to stress, and questions regarding biodiversity in ecological systems. Projects will focus on (1) epigenetic gene regulation; (2) parental control of gene expression; (3) response to viral infection; (4) metal intake in a type of Salmonella bacterium; (5) immune system response to a type of fungal pathogen; (6) vitamin dependent cell cycle progression; (7) phytoplankton communities in freshwater ecosystems; and (8) speciation response to changes in ploidy. This work will be shared with the scientific community through presentations at national conferences and publication with undergraduate co-authors in peer-reviewed journals.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

授予罗兹学院一个奖项，以支持收购荧光激活细胞分选仪（FACS），以加强罗兹学院和孟菲斯地区机构的教师研究和本科生培训。 该仪器允许从大群体中分离小颗粒，包括活细胞，并且基于非常特定的特征，例如颗粒大小或蛋白质的存在。 当细胞被分离时，可以收集关于每个细胞的非常详细的信息，并且可以进一步研究活细胞。添加的流式细胞仪将改变能力，以培养下一代科学家在罗兹教师主导的课程，指导研究，并在这个研究活跃的小学本科院校（PUI）推广计划。该仪器允许快速精确的分析和分离，非常强大，因此它将帮助科学家和本科生回答广泛的生物学问题，包括基因如何发挥作用，细胞如何应对压力以及有关生态系统中生物多样性的问题。项目将侧重于（1）表观遗传基因调控;（2）基因表达的亲代控制;（3）对病毒感染的反应;（4）一种沙门氏菌的金属摄入;（5）免疫系统对一种真菌病原体的反应;（6）依赖维生素的细胞周期进程;（7）淡水生态系统中的浮游植物群落;（8）对倍性变化的物种形成反应。 这项工作将通过在国家会议上的演讲和与本科生合著者在同行评审期刊上的出版物与科学界分享。该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估来支持。