Ocean Acidification Category 1 Collaborative Research: RUI: Synergistic Effects of Temperature and pH Variability on Physiology, Transcriptome and Proteome of Porcelain Crabs

海洋酸化一类合作研究：RUI：温度和pH变化对瓷蟹生理、转录组和蛋白质组的协同效应

• 批准号: 1041225
• 负责人: Jonathon Stillman
• 金额: $ 49.63万
• 依托单位: San Francisco State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1041225&HistoricalAwards=false
• 关键词: Ocean; Acidification; Category; Collaborative; Research

Marine environments are becoming more acidic as a result of increased atmospheric levels of carbon dioxide; this gas dissolves in sea water and is driving a process known as ocean acidification. However, organisms that live on the ocean floor (e.g. crabs, sea stars and oysters) experience, as a result of their biological activity, daily variations in their pH (acidity) that are greater than those caused by the increased levels of carbon dioxide in the atmosphere. This project addresses the comparative effects on porcelain crabs of increased ocean acidity (which changes slowly over years) with the daily variation in pH that these animals experience. The impacts of simultaneous changes in pH and temperature will be assessed, and it will be determined if early life history stages (i.e., crab larvae) are more sensitive to ocean acidification than adults. The responses that will be measured include calcification of the shell, metabolism, altered expression of genes and the modification of proteins. This research is expected to produce new and ecologically important information, across multiple levels of biological organization, about the impacts of ocean acidification on a coastal marine organism.Broader Impacts: This research will provide interdisciplinary training for graduate and undergraduate students in biochemistry, genomics, physiology and ecology. Students who are members of groups underrepresented in science will be recruited through programs at the San Francisco State University Student Enrichment Opportunity office, and through collaboration between Cal Poly and Hancock University. The investigators will incorporate this research in their undergraduate physiology classes, which emphasize active learning. A workshop on proteomics for researchers interested in ecological physiology will be conducted at Cal Poly San Luis Obispo. The investigators will present public lectures on ocean acidification and climate change, and write articles on these topics in the popular press. Teacher training workshops designed to enhance secondary education in climate change biology will be conducted at the Romberg Tiburon Center and Cal Poly San Luis Obispo.

由于大气中二氧化碳含量的增加，海洋环境正变得越来越酸；这种气体会溶解在海水中，导致海洋酸化。然而，生活在海底的生物（如螃蟹、海星和牡蛎）由于其生物活动，其pH值（酸度）的每日变化比大气中二氧化碳含量增加所造成的变化更大。这个项目解决了增加的海洋酸度（多年来变化缓慢）与这些动物所经历的pH值的日常变化对瓷蟹的比较影响。将评估pH值和温度同时变化的影响，并确定早期生活史阶段（即螃蟹幼虫）是否比成虫对海洋酸化更敏感。将被测量的反应包括外壳的钙化、新陈代谢、基因表达的改变和蛋白质的修饰。这项研究有望在生物组织的多个层面上，就海洋酸化对沿海海洋生物的影响产生新的和生态上重要的信息。更广泛的影响：本研究将为研究生和本科生提供生物化学、基因组学、生理学和生态学的跨学科培训。在科学领域未被充分代表的群体的学生将通过旧金山州立大学学生充实机会办公室的项目以及加州理工学院和汉考克大学的合作来招募。研究者将把这项研究纳入他们强调主动学习的本科生理学课程中。为对生态生理学感兴趣的研究人员举办的蛋白质组学研讨会将在加州理工学院圣路易斯奥比斯波分校举行。调查人员将发表关于海洋酸化和气候变化的公开演讲，并在大众媒体上撰写有关这些主题的文章。旨在加强气候变化生物学中等教育的教师培训讲习班将在隆伯格·蒂布隆中心和加州理工学院圣路易斯奥比斯波分校举行。