CAREER: Determining the molecular mechanisms of the Arabidopsis circadian clock: the role of the pseudo-response regulators

职业：确定拟南芥生物钟的分子机制：伪反应调节剂的作用

• 批准号: 1054243
• 负责人: Eva Farre
• 金额: $ 86.58万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2017-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1054243&HistoricalAwards=false
• 关键词: CAREER; Determining; molecular; mechanisms; Arabidopsis

The circadian clock is a self-sustained biological oscillator that enables organisms to both anticipate environmental changes and to coordinate different aspects of their physiology. In plants, the circadian clock is necessary for optimal growth and survival and regulates a wide range of physiological processes such as flowering and photosynthesis. The long-term goal of Dr. Farre's laboratory is to understand the design principles of circadian clocks as well as the role of the clock in plant growth and development. In particular, this project will help elucidate the molecular role of the morning expressed pseudo-response regulators PRR7 and PRR9 in the model plant Arabidopsis thaliana using molecular and biochemical approaches. These studies will define not only the molecular function of the PRRs in the circadian oscillator but also the direct role of clock components in the control of physiological responses in plants. Due to the architectural similarities between circadian networks in different organisms, this work will complement the analyses in other systems and advance our understanding of circadian clocks and their mechanisms to impact a large number of processes ranging from biomass production to human circadian disorders. Dr. Farre will also establish a two-year program at Michigan State University for students aimed at becoming secondary biology teachers to gain experience in scientific inquiry in a laboratory setting and to teach laboratory courses. Students will do a research internship in the Farre laboratory where they will work on their own research project associated with this award as well as establish a teaching unit that can be used in a high school class. Students will also be able to practice their teaching skills as teaching assistants of an undergraduate laboratory course.

生物钟是一种自我维持的生物振荡器，使生物体既能预测环境变化，又能协调其生理的不同方面。在植物中，生物钟是最佳生长和生存所必需的，并调节一系列的生理过程，如开花和光合作用。法尔博士实验室的长期目标是了解生物钟的设计原则，以及生物钟在植物生长和发育中的作用。特别是，该项目将有助于利用分子和生化方法阐明早晨表达的伪反应调节子PRR7和PRR9在模式植物拟南芥中的分子作用。这些研究不仅将确定PRRs在昼夜节律振荡器中的分子功能，而且还将确定时钟成分在控制植物生理反应中的直接作用。由于不同生物体的昼夜节律网络在结构上的相似性，这项工作将补充其他系统的分析，并促进我们对生物钟及其影响从生物量生产到人类昼夜节律紊乱的大量过程的机制的理解。法尔博士还将在密歇根州立大学设立一个为期两年的项目，面向希望成为中学生物学教师的学生，以便在实验室环境中获得科学探究的经验，并教授实验室课程。学生将在法尔实验室进行研究实习，在那里他们将致力于与该奖项相关的自己的研究项目，并建立一个可用于高中课堂的教学单元。学生还将能够作为本科实验室课程的助教来练习他们的教学技能。