Hochdruck-Perturbation des Ras-Signalwegs

Ras 信号通路的高压扰动

• 批准号: 200682527
• 负责人: Professor Dr. Roland Winter
• 金额: --
• 依托单位: Lehrstuhl für Physikalische Chemie I
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/200682527?language=en
• 关键词: Hochdruck; Perturbation; des; Ras; Signalwegs

We are planning to explore the effect of pressure modulation on the spatio-temporal patterns and reaction dynamics of membrane-associated signaling proteins of the Ras family using static and dynamic spectroscopic and microscopic techniques. In a first step, we plan to start with a minimum Ras signaling machinery using N-Ras and K-Ras and the presence of selected regulators and effectors (such as GTP/GDP, Sos, Raf) to explore the effect of pressure perturbation on the membrane partitioning, lateral organisation, and the reaction dynamics of the membrane-associated components. Based on these results on reconstituted membrane systems, we will then - in a later stage of the project - extend the use of the pressure perturbation approach for the modulation of reaction networks and identification of protein connectivity patterns also to in vivo systems.

我们计划利用静态和动态光谱和显微技术探索压力调节对Ras家族膜相关信号蛋白时空模式和反应动力学的影响。在第一步中，我们计划从使用N-Ras和K-Ras以及选定的调节剂和效应器（如GTP/GDP， Sos, Raf）的最小Ras信号机制开始，探索压力扰动对膜分配，横向组织和膜相关组分的反应动力学的影响。基于重组膜系统的这些结果，我们将在项目的后期阶段将压力扰动方法用于调节反应网络和鉴定蛋白质连接模式也扩展到体内系统。

项目成果

Pressure modulation of Ras-membrane interactions and intervesicle transfer.

• DOI: 10.1021/ja312671j
• 发表时间: 2013-04
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: S. Kapoor;Alexander Werkmüller;R. Goody;H. Waldmann;R. Winter

Revealing conformational substates of lipidated N-Ras protein by pressure modulation

• DOI: 10.1073/pnas.1110553109
• 发表时间: 2012-01-10
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Kapoor, Shobhna;Triola, Gemma;Winter, Roland
• 通讯作者: Winter, Roland

Exploring the structure and phase behavior of plasma membrane vesicles under extreme environmental conditions.

探索极端环境条件下质膜囊泡的结构和相行为

• DOI: 10.1039/c4cp05845c
• 发表时间: 2015
• 期刊: Physical chemistry chemical physics : PCCP
• 作者: J. Seeliger;N. Erwin;C. Rosin;M. Kahse;K. Weise;R. Winter
• 通讯作者: R. Winter

Packing Effects of N-Ras Binding to a DOPC Membrane – a Neutron Reflectivity and TIRF Spectroscopy High-Pressure Study

N-Ras 与 DOPC 膜结合的堆积效应 - 中子反射率和 TIRF 光谱高压研究

• DOI: 10.1515/zpch-2014-0546
• 发表时间: 2014
• 期刊: Zeitschrift für Physikalische Chemie
• 作者: M. Erlkamp;J.-F. Moulin;R. Winter;C. Czeslik
• 通讯作者: C. Czeslik