Structural investigations of VWF under flow by FTIR spectroscopy

通过 FTIR 光谱研究流动下 VWF 的结构

• 批准号: 200688970
• 负责人: Professor Dr. Klaus Gerwert
• 金额: --
• 依托单位: Lehrstuhl für Biophysik
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/200688970?language=en
• 关键词: Structural; investigations; VWF; under; flow

This proposal aims at the FTIR spectroscopic analysis of Von Willebrand Factor (vWF) structural plasticity under induced and controlled shear flow. vWF is the mechanosensoric protein which triggers primary hemostasis. The approach is based on the multidisciplinary design of a surface acoustic wave (SAW) driven flow cell device. This will enable the in detail structural analysis of vWF in a most native environment. Both polymeric full length, multidomain vWF complexes and single, functional domains will be analyzed. At least one surface of the flow device will be functionalizable. Thus, a specific immobilization of a vWF domain or a binding partner within adjustable shear flow will be used for binding studies. Further analysis of the enzymatic vWF cleavage with ADAMTS13 (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13) will give structural information about hemostasis regulation.

本研究的目的是利用傅立叶变换红外光谱法分析在诱导和控制剪切流动条件下的Von Willebrand因子（vWF）结构塑性。vWF是触发初级止血的机械感觉蛋白。该方法是基于表面声波（SAW）驱动的流动池设备的多学科设计。这将使vWF的详细结构分析在最天然的环境。将分析聚合全长、多结构域vWF复合物和单个功能结构域。流动装置的至少一个表面将是可官能化的。因此，vWF结构域或结合配偶体在可调节剪切流内的特异性固定将用于结合研究。用ADAMTS13（一种具有1型血小板反应蛋白基序的去整合素和金属蛋白酶，成员13）对vWF酶促裂解的进一步分析将提供有关止血调节的结构信息。