Elucidation of the unique conjugative DNA-translocation machinery of mycelial streptomycetes by biochemical analysis and real time imaging (A13)

通过生化分析和实时成像阐明菌丝链霉菌独特的接合 DNA 易位机制 (A13)

• 批准号: 200811073
• 金额: --
• 依托单位: Lehrstuhl für Mikrobiologie / Biotechnologie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/200811073?language=en
• 关键词: Elucidation; unique; conjugative; DNA; translocation

Streptomycetes developed a unique conjugative DNA-transfer system. In a process derived from the segregation of chromosomal DNA during bacterial cell division, a double-stranded DNA molecule is translocated and subsequently distributed to the mycelial compartments of the recipient. By reconstituting the DNA-transfer apparatus in vitro and by real time imaging of the DNA-transfer, we aim to elucidate the molecular processes directing Streptomyces conjugation.

链霉菌发展了一种独特的接合DNA转移系统。在细菌细胞分裂过程中染色体DNA分离的过程中，双链DNA分子易位，随后分布到受体的菌丝体区室。通过重建体外DNA转移装置和DNA转移的真实的时间成像，我们的目标是阐明指导链霉菌接合的分子过程。