Elucidation of the unique conjugative DNA-translocation machinery of mycelial streptomycetes by biochemical analysis and real time imaging (A13)

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Streptomycetes developed a unique conjugative DNA-transfer system. In a process derived from the segregation of chromosomal DNA during bacterial cell division, a double-stranded DNA molecule is translocated and subsequently distributed to the mycelial compartments of the recipient. By reconstituting the DNA-transfer apparatus in vitro and by real time imaging of the DNA-transfer, we aim to elucidate the molecular processes directing Streptomyces conjugation.