DISSERTATION RESEARCH: Genetic Architecture and Genomic Divergence During Ecological Speciation in Boechera stricta, a Wild Relative of Arabidopsis

论文研究：拟南芥野生近缘种 Boechera stricta 生态物种形成过程中的遗传结构和基因组分歧

• 批准号: 1110445
• 负责人: Thomas Mitchell-Olds
• 金额: $ 1.5万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2014-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1110445&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Genetic; Architecture; Genomic

This research aims to identify key factors contributing to the genetic differentiation within a species and the genetic mechanism underlying such differentiation. Using a North America native mustard plant species as model, preliminary study has found significant genotypic and phenotypic divergence between two subspecies. In order to study the genetic mechanism responsible for this differentiation, the investigators will generate a cross between the two subspecies and identify possible genes responsible for the phenotypic differentiation. Also, the direct contribution of genes and phenotypes to adaptation will be measured by growing the plants in their native environments. The investigators will further examine the genome-wide differentiation pattern between the two subspecies. Combining these results, the effect of local adaptation on the pattern of within-species genetic variation will be investigated empirically. This proposed research will advance understanding of patterns of within-species genetic diversity. Understanding the extent and driving factors of genetic variation will provide valuable information in various applications, such as identifying genetic factors underlying important traits in various species, including agricultural plants. The proposed project will be one of the first studies identifying possible genes responsible for important phenotypes, investigating the adaptive effect of these genes and phenotypes, and unifying these results with the investigation of population genomic divergence patterns. The utilization of a plant species in this research enables direct experimental manipulation on studied individuals. Finally, several undergraduate students will be trained with the interdisciplinary knowledge of ecology, population genetics, and genomics in this project.

本研究旨在确定导致物种内部遗传分化的关键因素和这种分化的遗传机制。以一种北美本地芥菜植物为模型，初步研究发现两个亚种之间存在显著的基因型和表型差异。为了研究这种分化的遗传机制，研究人员将在两个亚种之间产生一个杂交，并确定可能负责表型分化的基因。此外，基因和表型对适应性的直接贡献将通过植物在其原生环境中的生长来衡量。研究人员将进一步研究这两个亚种之间的全基因组分化模式。结合这些结果，实证研究了本地适应对种内遗传变异模式的影响。本研究将促进对种内遗传多样性模式的认识。了解遗传变异的程度和驱动因素将为各种应用提供有价值的信息，例如确定各种物种（包括农业植物）重要性状的遗传因素。该项目将是第一批确定重要表型的可能基因，研究这些基因和表型的适应效应，并将这些结果与群体基因组分化模式的研究相结合的研究之一。在本研究中利用植物物种可以对研究个体进行直接实验操作。最后，几名本科生将在本项目中接受生态学、群体遗传学和基因组学跨学科知识的培训。