Quantitative Analysis of Metabolic Networks and Flux Dynamics in Thermophilic Bacteria

嗜热细菌代谢网络和通量动力学的定量分析

• 批准号: 1120684
• 负责人: Maciej Antoniewicz
• 金额: $ 40.11万
• 依托单位: University of Delaware
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2016-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1120684&HistoricalAwards=false
• 关键词: Quantitative; Analysis; Metabolic; Networks; Flux

Thermus thermophilus is a potential host bacterium for the production of biofuels and other useful biochemicals. However, relatively little is known about its metabolism and the regulation of its metabolic activity. This project will characterize the metabolic network of this organism and quantify its metabolic flux phenotypes using classical steady-state techniques. In addition, techniques will be developed for dynamic metabolic flux analysis and metabolic network elucidation, requiring new tools and methods based on 13C-isotopomer analysis and tandem mass spectrometry. This project will 1) develop a new method of dynamic metabolic flux analysis, 2) provide improved techniques for validating metabolic network models, and 3) define optimized growth conditions for T. thermophilus. Broader Impacts: This project promises a major step forward in metabolic network analysis that will be broadly applicable to many organisms of biotechnological and biomedical importance. In addition, this project provides unique opportunities for graduate and undergraduate student education and training in an interdisciplinary environment comprising computational and experimental biology, metabolic flux analysis, mass spectrometry and metabolic network modeling.

嗜热栖热菌是生产生物燃料和其他有用生物化学品的潜在宿主细菌。然而，人们对其代谢及其代谢活性的调节知之甚少。该项目将描述该生物体的代谢网络，并使用经典稳态技术量化其代谢通量表型。此外，还将开发动态代谢流分析和代谢网络阐明技术，需要基于 13C 同位素异构体分析和串联质谱的新工具和方法。该项目将 1) 开发动态代谢流分析的新方法，2) 提供验证代谢网络模型的改进技术，以及 3) 定义嗜热链球菌的优化生长条件。更广泛的影响：该项目有望在代谢网络分析方面向前迈出重要一步，该分析将广泛适用于许多具有生物技术和生物医学重要性的生物体。此外，该项目还为研究生和本科生在跨学科环境中的教育和培训提供了独特的机会，包括计算和实验生物学、代谢流分析、质谱和代谢网络建模。