Analysis of RNA-metal ion interactions by solution Raman spectroscopy

通过溶液拉曼光谱分析 RNA-金属离子相互作用

• 批准号: 1121373
• 负责人: Michael Harris
• 金额: $ 45万
• 依托单位: Case Western Reserve University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-15 至 2014-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1121373&HistoricalAwards=false
• 关键词: Analysis; RNA; metal; ion; interactions

With this award, the Chemistry of Life Processes Program is supporting the research of Professor Michael Harris of the RNA Center at Case Western Reserve University to develop isotope-edited Raman spectroscopy as a new biophysical tool for the study of metal ions associated with backbone phosphate oxygens of RNA. Metal ion binding necessarily changes the chemical environment of neighboring phosphoryl oxygens, resulting in changes in the Raman spectrum. Signature signals in the Raman spectrum can in turn provide valuable information on the nature and position of the metal association. With isotope editing, these studies should allow for the experimental interrogation of specific metal-RNA interactions in solution, at the level of individual phosphates. The quantitative nature of the information gained makes it directly applicable to computational models being developed to describe the structure and energetics of RNA-metal ion binding.The Broader Impacts of the project will include the development of new tools for chemical biology based upon a combination of isotope-labeling (bioorganic chemistry) and Raman spectroscopy (biophysical chemistry). Success here may translate to broader applications of the technique in biophysical chemistry. The PI will incorporate advanced undergraduates into the research team, with a special emphasis on members of traditionally underrepresented groups in science. Student will have hands-on experience with state of the art instrumentation and participate in data analysis facilitated through small group learning exercises. Thus, an important component of the project is the integration of the research goals into education, providing a training opportunity for the next generation of scientists and engineers.This research project is being co-funded by the Biomolecular Dynamics, Structure and Function Cluster within the Molecular and Cellular Biosciences (MCB) Division of the BIO Directorate at NSF.

通过此奖项，《生命过程》计划的化学计划支持Case Western Reserve University RNA中心的Michael Harris教授的研究，以开发同位素编辑的拉曼光谱，作为一种新的生物物理工具，用于研究与RNA骨链磷酸盐氧相关的金属离子。 金属离子结合必然会改变相邻磷酸化氧的化学环境，从而导致拉曼光谱的变化。 拉曼频谱中的签名信号反过来可以提供有关金属关联性质和位置的宝贵信息。 通过同位素编辑，这些研究应允许在单个磷酸盐水平的溶液中对特定金属RNA相互作用进行实验审查。 获得的信息的定量性质使其直接适用于正在开发的计算模型，以描述RNA - 金属离子结合的结构和能量学。该项目的更广泛影响包括基于同位素标记（生物有机化学）和Raman分类化学（Raman Chepstromcoscopy（Biophysical Chemysical）的组合，开发了新的化学生物学工具。 这里的成功可能转化为该技术在生物物理化学中的更广泛应用。 PI将把高级本科生纳入研究团队，并特别强调传统上代表性不足的科学群体成员。 学生将在最先进的仪器方面拥有动手经验，并通过小组学习练习参与促进的数据分析。因此，该项目的一个重要组成部分是将研究目标整合到教育中，为下一代科学家和工程师提供了培训机会。该研究项目由分子和细胞生物学家（MCB）内的生物分子动态，结构和功能集群共同资助。