MRI: Acquisition of an Ion Torrent PGM Sequencer for Research and Education

MRI：购买 Ion Torrent PGM 测序仪用于研究和教育

• 批准号: 1125982
• 负责人: Michael Thomas
• 金额: $ 19.44万
• 依托单位: Idaho State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1125982&HistoricalAwards=false
• 关键词: MRI; Acquisition; Ion; Torrent; PGM

This Major Research Instrumentation (MRI) award funds the acquisition of an Ion Torrent Personal Genome Machine (Ion PGM) sequencer to enable next-generation sequencing at the Molecular Research Core Facility of Idaho State University (ISU). Next-generation sequencing technology enables experiments that were, until recently, virtually impossible to perform at institutions the size of ISU, and provides a way for the researcher to determine the exact sequence of all the DNA and RNAs in an organism at a given time. Understanding how organisms function and interact with their environment in normal and perturbed states is a major goal of biological and medical researchers. The Ion PGM at ISU will enable researchers to explore physiology and genomics of individual microbes and complex communities, with emphasis on bioenergy and bioremediation; reconstruct human colonization and adaptation in Pacific Island environments using phylogeographic and demographic models; identify variation in genes for fiber, antioxidants and fatty acids in crop plants; identify genetic components of plant ecophysiological responses to climate change; identify genes associated with microbial radiation resistance; and profile gene expression patterns in model organisms exposed to psychoactive pharmaceutical contamination in aquatic systems. The results from these studies will be presented at scientific conferences, published in peer-reviewed journals and deposited in public databases for the benefit of the biological research community. Furthermore, through their research activities and mentorship of graduate and undergraduate students, the co-PIs are deeply committed to providing training opportunities for Idahoans. The ISU Molecular Research Core Facility has a long history of reaching out to regional educational K-12 institutions, undergraduate institutions, Federal research labs and biotechnology companies, to encourage broader participation in science and promote the training and development of the next generation of research scientists.

该主要研究仪器（MRI）奖资助收购离子激流个人基因组机（Ion PGM）测序仪，以实现爱达荷州立大学（ISU）分子研究核心设施的下一代测序。新一代测序技术使实验成为可能，直到最近，几乎不可能在ISU这样规模的机构中进行，并为研究人员提供了一种方法来确定在给定时间内生物体中所有DNA和rna的确切序列。了解生物体在正常状态和受干扰状态下如何发挥作用并与环境相互作用是生物学和医学研究人员的主要目标。ISU的离子PGM将使研究人员能够探索个体微生物和复杂群落的生理学和基因组学，重点是生物能源和生物修复；利用系统地理学和人口学模型重建人类在太平洋岛屿环境中的殖民和适应；鉴定作物中纤维、抗氧化剂和脂肪酸基因的变异；确定植物对气候变化生态生理响应的遗传成分；鉴定与微生物辐射抗性相关的基因；并在水生系统中暴露于精神活性药物污染的模式生物中描述基因表达模式。这些研究的结果将在科学会议上发表，在同行评议的期刊上发表，并存入公共数据库，以造福生物研究界。此外，通过他们的研究活动和对研究生和本科生的指导，合作项目负责人坚定地致力于为爱达荷人提供培训机会。ISU分子研究核心设施与地区教育机构K-12、本科院校、联邦研究实验室和生物技术公司有着悠久的历史，以鼓励更广泛的科学参与，促进下一代研究科学家的培训和发展。