MRI: Acquisition of a Mass Spectrometer

有了这项来自主要研究仪器（MRI）计划的奖项，圣克拉拉大学的Michael Carrasco教授和同事Amelia Fuller将获得电喷雾电离串联（ESI-MS/MS）质谱系统。该奖项将加强各级的研究培训和教育，特别是在以下领域：（a）识别模拟蛋白质的折叠体，（B）保护免受蛋白质水解的肽的化学选择性糖基化，（c）糖脂肽的合成，（d）糖肽和糖蛋白的适体选择，（e）北极光心包器官释放生物胺的表征，（f）表征蛋白质和银纳米颗粒之间的相互作用，以及（g）鉴定卟啉组装体的配体结合性质。质谱仪（MS）用于通过在样品中的分子成分被电离并被质谱仪检测之后测量样品中的分子成分的质量来鉴定样品的化学组成。该仪器将高分辨率液相色谱系统与质谱的质量分析能力相结合。液相色谱仪位于光谱仪的入口处，将混合物分离成其分子组分。然后这些组分流入质谱仪，在质谱仪中使用电喷雾源将其电离。串联质谱仪（MS/MS）涉及多个步骤，在阶段之间发生某种形式的碎片化。这样，它们的质量和碎片的质量就被测量出来了。这些仪器不仅将用于研究，还将用于实验室课程，以培训大量学生使用这一重要的分析技术。

With this award from the Major Research Instrumentation (MRI) program, Professor Michael Carrasco from Santa Clara University and colleague Amelia Fuller will acquire an electrospray inonization tandem (ESI-MS/MS) mass spectrometric system. The award will enhance research training and education at all levels, especially in areas such as (a) identification of foldamers that mimic proteins, (b) chemoselective glycosylation of peptides that protection from proteolysis, (c) synthesis of glycolipopeptides, (d) aptamer selection for glycopeptides and glycoproteins, (e) characterization of release of biogenic amines from the pericardial organs of cancer borealis, (f) characterization of interactions between proteins and silver nanoparticles, and (g) identification of the ligand-binding properties of porphyrin assemblies.Mass spectrometers (MS) are used to identify the chemical composition of a sample by measuring the mass of the molecular constituents in the sample after they are ionized and detected by the mass spectrometer. This instrument couples a high-resolution liquid chromatography system with the mass analysis ability of mass spectrometry. A liquid chromatograph located at the mouth of the spectrometer separates mixtures into their molecular components. These components then flow into the mass spectrometer where they are ionized using an electrospay source. The tandem mass spectrometer (MS/MS) involves multiple steps with some form of fragmentation occurring between stages. In this way their masses, and those of their fragments, are measured. The instrumentation will be used not only for research but also in laboratory courses to train significant numbers of students in the use of this important analytical technique.