Development of an Electron Tunneling Based Nanochannel System for DNA Sequencing

基于电子隧道的 DNA 测序纳米通道系统的开发

• 批准号: 1128660
• 负责人: Steve Tung
• 金额: $ 35.97万
• 依托单位: University of Arkansas
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-04-01 至 2016-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1128660&HistoricalAwards=false
• 关键词: Development; Electron; Tunneling; Based; Nanochannel

The objective of this program is to design and fabricate a silicon nanochannel system with embedded nanoelectrodes for DNA sequencing. The sequencing principle is based on detecting the electron tunneling property of individual nucleotides of a single-stranded DNA as it translocates through a nanochannel. The nanochannel will be fabricated based on MEMS microfabrication and AFM nanolithography. The intellectual merit of the program is the integration of the latest DNA sensing principle with advanced AFM techniques in realizing a label-free sequencing device that can provide fast and specific detection. The development of this rapid DNA sequencing technique is transformative. The proposed project will explore several novel areas such as AFM machining of nanofluidic channels, optimization of DNA electron tunneling sensitivity, and specificity of functionalized nanoelectrodes towards DNA bases. Results of the proposed project are expected to impact future designs of nanofluidic based nanomedicine systems. The broader impacts of the program are the advancement of the knowledge of bionanotechnology and the development of an important tool for both biomedical research and clinical diagnostics. Additionally, the proposed research will also generate diversified learning experience for students involved in the project, who will have a rare opportunity to be cross-trained in nanofabrication and DNA processing. The fabrication techniques and recipes developed by the project are expected to greatly enhance the core capability of the multi-user cleanroom facility at the University of Arkansas in areas such as MEMS, BioMEMS, and NEMS.

本计画的目的是设计并制作一个可用于DNA定序的内嵌奈米电极矽奈米通道系统。测序原理是基于检测单链DNA的单个核苷酸的电子隧穿性质，因为它通过纳米通道易位。纳米通道将基于MEMS微加工和AFM纳米光刻来制造。该计划的智力价值是将最新的DNA传感原理与先进的AFM技术相结合，实现了一种可以提供快速和特异性检测的无标记测序设备。这种快速DNA测序技术的发展是变革性的。拟议的项目将探索几个新的领域，如纳米流体通道的AFM加工，DNA电子隧穿灵敏度的优化，以及功能化纳米电极对DNA碱基的特异性。预计该项目的结果将影响基于纳米流体的纳米医学系统的未来设计。该计划的更广泛影响是生物纳米技术知识的进步以及生物医学研究和临床诊断的重要工具的开发。此外，拟议的研究还将为参与该项目的学生提供多样化的学习经验，他们将有难得的机会在纳米纤维和DNA处理方面进行交叉培训。该项目开发的制造技术和配方有望大大提高阿肯色州大学多用户洁净室设施在MEMS、BioMEMS和NEMS等领域的核心能力。