Post-translation regulation of small RNA-guided gene silencing pathways (B03/B04)

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MicroRNA (miRNA)-guided gene silencing is a central process in almost all cellular pathways and therefore affected in many diseases. MiRNAs guide Argonaute (Ago) and TNRC6 proteins to complementary sites on target mRNA leading to gene silencing. This process is embedded into extended regulatory networks including protein interactors as well as post-translational modification pathways. In project B3, we will investigate signaling pathways that affect gene silencing and functionally characterize protein interaction partners of Ago and TNRC6, which we have identified during the pervious funding period. We have already established biochemical purification strategies to isolate Ago- and TNRC6-containg mRNA complex. This will be further optimized and used for structural studies using cryo-electron-microscopy.