Post-translation regulation of small RNA-guided gene silencing pathways (B03/B04)

小RNA引导的基因沉默途径的翻译后调控（B03/B04）

• 批准号: 202862799
• 金额: --
• 依托单位: Lehrstuhl für Biochemie I
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/202862799?language=en
• 关键词: Post; translation; regulation; small; RNA

MicroRNA (miRNA)-guided gene silencing is a central process in almost all cellular pathways and therefore affected in many diseases. MiRNAs guide Argonaute (Ago) and TNRC6 proteins to complementary sites on target mRNA leading to gene silencing. This process is embedded into extended regulatory networks including protein interactors as well as post-translational modification pathways. In project B3, we will investigate signaling pathways that affect gene silencing and functionally characterize protein interaction partners of Ago and TNRC6, which we have identified during the pervious funding period. We have already established biochemical purification strategies to isolate Ago- and TNRC6-containg mRNA complex. This will be further optimized and used for structural studies using cryo-electron-microscopy.

microRNA（miRNA）引导的基因沉默是几乎所有细胞通路的中心过程，因此在许多疾病中受到影响。MiRNA将Argonaute（Ago）和TNRC6蛋白引导至靶mRNA上的互补位点，导致基因沉默。这一过程被嵌入到扩展的调控网络中，包括蛋白质相互作用物以及翻译后修饰途径。在项目B3中，我们将研究影响基因沉默的信号通路，并在功能上表征Ago和TNRC6的蛋白质相互作用伙伴，这是我们在前一个资助期间确定的。我们已经建立了生物化学纯化策略来分离含有Ago和TNRC6的mRNA复合物。这将进一步优化，并用于使用冷冻电子显微镜的结构研究。