Evaluating Beneficial Bacteria Function Following Engineered Nanoparticle Exposure

评估工程纳米颗粒暴露后的有益细菌功能

• 批准号: 1152931
• 负责人: Christy Haynes
• 金额: $ 42.01万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-02-01 至 2016-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1152931&HistoricalAwards=false
• 关键词: Evaluating; Beneficial; Bacteria; Function; Following

The Environmental Chemical Sciences (ECS) program of the Division of Chemistry will support the research program of Prof. Christy Haynes of the University of Minnesota. Prof. Haynes and her students will study the interaction of beneficial bacteria with engineered nanoparticles and assess the role that simulated natural water has on nanoparticle characteristics and bacterial impact. Initial efforts will focus on characterizing nanoparticle behavior in simulated natural water by studying nanoparticle dispersity and stability in the presence of natural organic matter (NOM) while also working to understand transformations the NOM experiences after nanoparticle introduction. Furthering mechanistic understanding of bacteria-nanoparticle interactions, the next stage of the study will address how nanoparticle presence influences S. oneidensis function, namely biofilm formation and flavin secretion. These functions have numerous constructive impacts on the ecosystem, and disruption by nanoparticles would be significant. The study addresses the critical gap in ecological toxicity knowledge surrounding the interaction of commonly used engineered nanoparticles and the beneficial bacteria, S. oneidensis. The nanoparticles chosen for investigation, TiO2 and Ag, are likely candidates for unintentional release into the aquatic ecosystem because of their common use in consumer products. Within the food web, the interaction of engineered nanoparticles with beneficial bacteria is important based on the constructive function of bacteria itself as well as their interaction with other organisms. It is critical, therefore, to consider how ecologically important organisms are impacted by these nanomaterials. S. oneidensis is an ideal model beneficial bacteria because they are widely distributed, geographically and throughout the aquatic ecosystem. As a part of the project, Prof. Haynes and her students will develop a high school laboratory activity titled, "Nanoparticle Toxicity in Brine Shrimp" where students will get a hands-on experience examining the effects of noble metal nanoparticles on an aquatic invertebrate. They will learn concepts from chemistry, microbiology, and statistics, while exploring this cutting edge topic. The research project will provide excellent educational opportunities for students, including some from underrepresented groups, desiring to work at the forefront of environmental science.

化学系的环境化学科学（ECS）计划将支持明尼苏达大学Christy Haynes教授的研究计划。Haynes教授和她的学生将研究有益细菌与工程纳米颗粒的相互作用，并评估模拟天然水对纳米颗粒特性和细菌影响的作用。最初的努力将集中在表征纳米粒子在模拟天然水中的行为，通过研究纳米粒子在天然有机物（NOM）存在下的分散性和稳定性，同时也致力于了解纳米粒子引入后NOM经历的转变。为了进一步了解细菌-纳米颗粒相互作用的机理，下一阶段的研究将解决纳米颗粒的存在如何影响S。oneidensis功能，即生物膜形成和黄素分泌。这些功能对生态系统有许多建设性的影响，纳米颗粒的破坏将是显著的。这项研究解决了生态毒性知识的关键差距，围绕常用的工程纳米粒子和有益细菌，S。oneidensis。选择用于调查的纳米颗粒，二氧化钛和银，可能是无意中释放到水生生态系统中的候选人，因为它们在消费品中的常见用途。在食物网中，基于细菌本身的建设性功能以及它们与其他生物体的相互作用，工程纳米颗粒与有益细菌的相互作用非常重要。因此，至关重要的是要考虑这些纳米材料对生态重要生物的影响。S. oneidensis是一种理想的模式有益细菌，因为它们在地理上和整个水生生态系统中广泛分布。作为该项目的一部分，Haynes教授和她的学生将开发一个名为“盐水虾中的纳米颗粒毒性”的高中实验室活动，学生将获得实践经验，检查贵金属纳米颗粒对水生无脊椎动物的影响。他们将学习化学，微生物学和统计学的概念，同时探索这个前沿话题。该研究项目将为学生提供良好的教育机会，包括一些来自代表性不足的群体，希望在环境科学的前沿工作。