Integrated OMICS Study of the Beneficial Effects of Egg Shell Membrane-derived Resistant Protein on Intestinal Bacteria

蛋壳膜衍生抗性蛋白对肠道细菌有益作用的综合组学研究

• 批准号: 20F20401
• 负责人: 加藤 久典
• 金额: $ 1.47万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2020
• 资助国家: 日本
• 起止时间: 2020-11-13 至 2022-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20F20401/
• 关键词: registant protein; eggshel membrane; microbiota; sake; IgA; high fat diet; eggshell membrane; Profiber; resistant proteins; high-fat diet;; metabolic dysfunctions; gut microbiota; metabolites

Consumption of resistant proteins (RP: non-digestible proteins) has been reported to exert beneficial functions. However, the involvement of gut microbiota in such beneficial effects is not fully elucidated. Previous studies have shown eggshel membrane (ESM) and sake cake (Profiber: PFB) contain a large amount of RP. Here, I performed the first animal experiment to investigate the effects of dietary ESM or PFB on metabolic dysfunctions and gut dysbiosis induced by high-fat diet (HFD).A total of 48 C57BL6J male mice was assigned randomly to the following six dietary groups: (1) Control diet (Ctrl), (2) high-fat diet (HFD), (3) HFD with 8% ESM (HESM), (4) HFD with 8% ESM+ antibiotics mix (ESMAbx), (5) HFD with 30% PFB (HPFB), (6) HFD with 30% PFB + antibiotics mix (PFBAbx). Compared with Ctrl group, mice fed an HFD showed increased body weight, visceral fat mass, liver weight, liver triglycerides, and liver cholesterol. Compared with the HFD group, the mice body weight gains were reduced in both of HESM and ESMAbx groups. However, the mice body weight gains were not affected in HPFB and PFBAbx groups compared that in the HFD group. The oral glucose tolerance test was not affected in these treatment groups. PFB treatment reduced the level of cholesterol in serum and liver. Compared with the Ctrl group, HFD showed increased level of immunoglobulin (IgA) in colon content. ESM treatment did not affect IgA level in colon content compared with that of HFD group. PFB treatment was shown to slightly increase the IgA level in colon content.

据报道，抗性蛋白质（RP：不可消化的蛋白质）的消耗发挥有益的功能。然而，肠道微生物群参与这些有益作用的情况尚未完全阐明。以往的研究表明，蛋壳膜（ESM）和清酒饼（Profiber：PFB）含有大量的RP。在此，我进行了第一个动物实验，以研究膳食ESM或PFB对高脂饮食（HFD）诱导的代谢功能障碍和肠道生态失调的影响。总共48只C57 BL 6J雄性小鼠被随机分配到以下6个膳食组：（1）对照饮食（Ctrl），（2）高脂肪饮食（HFD），（3）具有8%ESM的HFD（HESM），（4）具有8%ESM+抗生素混合物的HFD（ESMAbx），（5）具有30%PFB的HFD（HPFB），（6）HFD +30%PFB+抗生素混合物（PFBAbx）。与对照组相比，喂食HFD的小鼠显示体重、内脏脂肪量、肝脏重量、肝脏甘油三酯和肝脏胆固醇增加。与HFD组相比，HESM和ESMAbx组小鼠体重增加减少。然而，与HFD组相比，HPFB和PFBAbx组的小鼠体重增加不受影响。这些治疗组的口服葡萄糖耐量试验未受影响。PFB处理降低血清和肝脏中的胆固醇水平。与对照组相比，HFD组结肠内容物中免疫球蛋白（伊加）水平升高。与HFD组相比，ESM治疗对结肠内容物中伊加水平无影响。PFB处理显示出略微增加结肠内容物中的伊加水平。