Integrated OMICS Study of the Beneficial Effects of Egg Shell Membrane-derived Resistant Protein on Intestinal Bacteria

蛋壳膜衍生抗性蛋白对肠道细菌有益作用的综合组学研究

• 批准号: 20F20401
• 负责人: 加藤 久典
• 金额: $ 1.47万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2020
• 资助国家: 日本
• 起止时间: 2020-11-13 至 2022-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20F20401/
• 关键词: registant protein; eggshel membrane; microbiota; sake; IgA; high fat diet; eggshell membrane; Profiber; resistant proteins; high-fat diet;; metabolic dysfunctions; gut microbiota; metabolites

Consumption of resistant proteins (RP: non-digestible proteins) has been reported to exert beneficial functions. However, the involvement of gut microbiota in such beneficial effects is not fully elucidated. Previous studies have shown eggshel membrane (ESM) and sake cake (Profiber: PFB) contain a large amount of RP. Here, I performed the first animal experiment to investigate the effects of dietary ESM or PFB on metabolic dysfunctions and gut dysbiosis induced by high-fat diet (HFD).A total of 48 C57BL6J male mice was assigned randomly to the following six dietary groups: (1) Control diet (Ctrl), (2) high-fat diet (HFD), (3) HFD with 8% ESM (HESM), (4) HFD with 8% ESM+ antibiotics mix (ESMAbx), (5) HFD with 30% PFB (HPFB), (6) HFD with 30% PFB + antibiotics mix (PFBAbx). Compared with Ctrl group, mice fed an HFD showed increased body weight, visceral fat mass, liver weight, liver triglycerides, and liver cholesterol. Compared with the HFD group, the mice body weight gains were reduced in both of HESM and ESMAbx groups. However, the mice body weight gains were not affected in HPFB and PFBAbx groups compared that in the HFD group. The oral glucose tolerance test was not affected in these treatment groups. PFB treatment reduced the level of cholesterol in serum and liver. Compared with the Ctrl group, HFD showed increased level of immunoglobulin (IgA) in colon content. ESM treatment did not affect IgA level in colon content compared with that of HFD group. PFB treatment was shown to slightly increase the IgA level in colon content.

据报道，耐药蛋白（RP：不可消化的蛋白质）的消耗据报道发挥有益的功能。但是，肠道菌群参与这种有益作用并未完全阐明。先前的研究表明，蛋壳膜（ESM）和清酒蛋糕（Profiber：PFB）包含大量RP。在这里，我进行了第一个动物实验，研究了饮食中的ESM或PFB对高脂饮食（HFD）诱导的代谢功能障碍和肠道疾病的影响。总共将48个C57BL6J雄性小鼠随机分配为以下六个饮食组：（1）对照饮食（1）对照饮食（Ctrl），（2）高饮食（2）HFM（3％）HFM HFM HFM HFM（HFM），HFM HFM（3％）HFM HFM（HFM），HFM HFM（3％）HFM HFM（HFM）， （HESM），（4）HFD，具有8％ESM +抗生素混合物（ESMABX），（5）HFD，HFD为30％PFB（HPFB），（6）HFD，HFD，30％PFB +抗生素混合物（PFBABX）。与CTRL组相比，喂养HFD的小鼠体重增加，内脏脂肪量，肝脏重量，肝甘油三酸酯和肝胆固醇。与HFD组相比，HESM和ESMABX组的小鼠体重增加均减少。但是，在HPFB和PFBABX组中，小鼠体重增加不受影响。在这些治疗组中，口服葡萄糖耐量测试不受影响。 PFB治疗降低了血清和肝脏中胆固醇的水平。与CTRL组相比，HFD在结肠含量中显示出免疫球蛋白（IGA）的水平升高。与HFD组相比，ESM治疗不会影响结肠含量的IgA水平。 PFB治疗显示出结肠含量的IgA水平略有增加。