Integrated OMICS Study of the Beneficial Effects of Egg Shell Membrane-derived Resistant Protein on Intestinal Bacteria

据报道，抗性蛋白（RP：不可消化蛋白）的消耗发挥了有益的作用。然而，肠道微生物群在这种有益作用中的作用尚未完全阐明。以往的研究表明，蛋壳膜（ESM）和清酒饼（Profiber: PFB）中含有大量的RP。在这里，我进行了第一个动物实验来研究膳食ESM或PFB对高脂肪饮食（HFD）诱导的代谢功能障碍和肠道生态失调的影响。将48只C57BL6J雄性小鼠随机分为6个饮食组：(1)控制饮食（Ctrl），(2)高脂饮食（HFD）， (3) HFD加8% ESM (HESM), (4) HFD加8% ESM+抗生素组合（ESMAbx）， (5) HFD加30% PFB (HPFB), (6) HFD加30% PFB +抗生素组合（PFBAbx）。与对照组相比，饲喂HFD的小鼠体重、内脏脂肪量、肝脏重量、肝脏甘油三酯和肝脏胆固醇均增加。与HFD组相比，HESM和ESMAbx组小鼠的体重增加均有所减少。然而，与HFD组相比，HPFB和PFBAbx组小鼠体重增加不受影响。这些治疗组的口服糖耐量试验不受影响。PFB治疗降低了血清和肝脏中的胆固醇水平。与对照组相比，HFD组结肠内容物中免疫球蛋白（IgA）水平升高。与HFD组相比，ESM治疗未影响结肠内容物中IgA水平。PFB处理显示结肠内容物中IgA水平略有增加。

Consumption of resistant proteins (RP: non-digestible proteins) has been reported to exert beneficial functions. However, the involvement of gut microbiota in such beneficial effects is not fully elucidated. Previous studies have shown eggshel membrane (ESM) and sake cake (Profiber: PFB) contain a large amount of RP. Here, I performed the first animal experiment to investigate the effects of dietary ESM or PFB on metabolic dysfunctions and gut dysbiosis induced by high-fat diet (HFD).A total of 48 C57BL6J male mice was assigned randomly to the following six dietary groups: (1) Control diet (Ctrl), (2) high-fat diet (HFD), (3) HFD with 8% ESM (HESM), (4) HFD with 8% ESM+ antibiotics mix (ESMAbx), (5) HFD with 30% PFB (HPFB), (6) HFD with 30% PFB + antibiotics mix (PFBAbx). Compared with Ctrl group, mice fed an HFD showed increased body weight, visceral fat mass, liver weight, liver triglycerides, and liver cholesterol. Compared with the HFD group, the mice body weight gains were reduced in both of HESM and ESMAbx groups. However, the mice body weight gains were not affected in HPFB and PFBAbx groups compared that in the HFD group. The oral glucose tolerance test was not affected in these treatment groups. PFB treatment reduced the level of cholesterol in serum and liver. Compared with the Ctrl group, HFD showed increased level of immunoglobulin (IgA) in colon content. ESM treatment did not affect IgA level in colon content compared with that of HFD group. PFB treatment was shown to slightly increase the IgA level in colon content.