Magnetic bead based preconcentration and sampling for biosensing in complex media

Hesketh 1159726生物测定中的一个关键挑战是从复杂基质中捕获靶标。我们建议开发一种新的样品制备和预浓缩微流控技术，从复杂的混合物，使用功能化的磁珠引导的软磁结构和旋转磁场混合和收集特定的试剂。将建立一个中尺度计算模式，以研究捕获过程的关键特征。长期的目标是开发一个实用的紧凑型便携式预浓缩和病原体纯化系统的复杂的混合物，重要的食品和环境安全，可应用于广泛的assays.Intellectual优点：一个基本的限制与磁珠采样的目标捕获效率低。磁引导样品预处理微流控系统导致高捕获效率的最佳操作条件将被建立为目标相对于珠的大小，相对流量和珠速度，目标和珠之间的粘附力和其他factors.Broader影响：总体目标是证明从一个复杂的矩阵有效的采样真实的世界样品的相关食品安全。利用旋转外部磁场和微尺度软磁特征，可以操纵磁珠的移动和放置以改善靶捕获。这种新方法将对许多类型的生物测定产生重大影响，其中目标分析物浓度低或样品基质复杂，包括临床测定。跨学科和教育影响：这种基于珠的采样和混合的跨学科研究需要机械工程，电气工程和食品科学的专业知识。应用优化的实验流体装置检测食品样品中的病原体在食品安全中心在格鲁吉亚大学将被证明。这项工作将对许多类型的生物测定产生重大影响，包括临床测定，其中靶标在复杂基质中的浓度较低。该项目将涉及并培训研究生和本科生、高中教师和高中学生。

Hesketh1159726A key challenge in bioassays is the capturing of a target from a complex matrix. We propose to develop a new sample preparation and pre-concentration microfluidic technique from complex mixtures that uses functionalized magnetic beads guided by soft-magnetic structures and rotating magnetic fields to mix and collect specific reagents. A mesoscale computational model will be developed to study the key characteristics of the capture process. The long term objective is to develop a practical compact portable pre-concentration and pathogen purification system for complex mixtures important for food and environmental safety that can be applied to a wide range of assays.Intellectual Merit: A fundamental limitation of sampling with magnetic beads is low efficiency of target capture. Optimal operation conditions for magnetic-guided sample preconditioning microfluidic systems leading to high capture efficiency will be established as a function of the size of the target relative the bead, the relative flow and bead velocity, adhesion forces between the target and bead and other factors.Broader Impact: The overall objective is to demonstrate efficient sampling from a complex matrix real world sample of relevance to food safety. Utilizing rotating external magnetic fields and microscale soft magnetic features the movement and placement of magnetic beads can be manipulated to improve target capture. This novel method will have significant impact on many types of bioassays where a target analyte is at low concentration or where the sample matrix is complex including clinical assays.Interdisciplinary and Education Impact: This interdisciplinary research on bead based sampling and mixing requires expertise in mechanical engineering, electrical engineering and food science. Application of the optimized experimental fluidic device to the detection of pathogens in food samples at the Center for Food Safety at University of Georgia will be demonstrated. This work will have significant impact on many types of bioassays including clinical assays, where a target is at low concentration in a complex matrix is complex. This project will involve and train graduate and undergraduate students, high school teachers and high school students.