A multifunctional neural stem cell delivery system for brain tissue regeneration

用于脑组织再生的多功能神经干细胞输送系统

• 批准号: 1160122
• 负责人: Jianjun Guan
• 金额: $ 34.81万
• 依托单位: Ohio State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-06-15 至 2016-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1160122&HistoricalAwards=false
• 关键词: multifunctional; neural; stem; cell; delivery

1160122 GuanThe objective of this proposal is to create a novel neural stem cell (NSC) delivery system that will ultimately improve efficacy of the NSC transplantation into brain for brain tissue regeneration. The system will not only passively protect NSCs from immune attack, but also actively stimulate NSC survival and differentiation under hypoxic condition, a typical condition at the transplantation site. NSC therapy holds a great potential to treat brain diseases. However, direct NSC transplantation often results in a high rate of cell death, caused primarily by host immune attack and hypoxic condition at the transplantation site. A potential engineering solution is to use a biomaterial-based NSC delivery system to protect the transplanted cells. Yet, the efficacy of this approach is limited due to inefficient immunoprotection, limited cell-biomaterial interaction, and hypoxic condition. The proposed work will address these issues by encapsulating NSCs in immunoprotection capsules that will effectively block both immune proteins and pro-inflammatory cytokines. The capsules will also possess a microenvironment to stimulate the differentiation of NSCs into neurons. To actively stimulate NSC survival under hypoxic condition, cells will be continuously oxygenated. The proposed studies will generate broader impacts on cell transplantation for disease treatment and foster three levels of education and outreach activities. Technically, the research has the potential to advance the current design strategy for cell transplantation systems and significantly improve efficacy of cell transplantation-based disease treatment. It also enables a multifaceted education and outreach plan, including exposing high school students to polymers and biotechnologies; engaging high school female students in science and engineering; and providing underrepresented/minority graduate students with solid training in the biotechnology field and will educate a new generation of biomedical scientists and engineers.

1160122 Guan该建议的目的是创造一种新的神经干细胞（NSC）输送系统，该系统将最终提高NSC移植到脑中用于脑组织再生的功效。该系统不仅会被动地保护NSC免受免疫攻击，而且会主动地刺激NSC在缺氧条件下的存活和分化，这是移植部位的典型条件。NSC疗法在治疗脑部疾病方面具有巨大潜力。然而，直接NSC移植通常导致高细胞死亡率，主要由宿主免疫攻击和移植部位的缺氧条件引起。一个潜在的工程解决方案是使用基于生物材料的NSC递送系统来保护移植的细胞。然而，这种方法的功效是有限的，由于低效的免疫保护，有限的细胞-生物材料相互作用，和缺氧条件。拟议的工作将通过将神经干细胞封装在免疫保护胶囊中来解决这些问题，这些胶囊将有效地阻断免疫蛋白和促炎细胞因子。胶囊还将具有刺激NSC分化成神经元的微环境。为了积极刺激NSC在缺氧条件下的存活，细胞将被持续充氧。拟议的研究将对用于疾病治疗的细胞移植产生更广泛的影响，并促进三级教育和外联活动。从技术上讲，该研究有可能推进当前细胞移植系统的设计策略，并显着提高基于细胞移植的疾病治疗的疗效。它还促成了一个多方面的教育和外联计划，包括让高中生接触聚合物和生物技术;让高中女生参与科学和工程;为代表性不足/少数民族的研究生提供生物技术领域的扎实培训，并将教育新一代生物医学科学家和工程师。