Instructive scaffolds capable of simultaneously regenerating multiple periodontal structures

能够同时再生多个牙周结构的指导性支架

• 批准号: 1708956
• 负责人: Jianjun Guan
• 金额: $ 39万
• 依托单位: Ohio State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-15 至 2019-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1708956&HistoricalAwards=false
• 关键词: Instructive; scaffolds; capable; simultaneously; regenerating

Non-Technical Summary:Scaffolds have been widely used for tissue regeneration. Yet it remains challenging to design scaffolds that simultaneously induce endogenous regeneration of multiple tissues with inherently low regenerative potential, such as periodontal tissues and musculoskeletal tissues. Current scaffolds lack the capability to concurrently recruit different cell types to the diseased sites and promoting them to develop into tissues. This project will design scaffolds that address this current challenge. The scaffolds will be tested using periodontal cells as model cells. The proposed studies will provide a fundamental understanding of the effects of scaffold physical and biological properties on the recruitment of several cell types at the same time, and directing them to regenerate into corresponding tissues. The outcomes of this project will guide the development of scaffolds for simultaneous regeneration of multiple tissue structures. The proposed research is integrated with a multifaceted education and outreach plan. It includes (1) mentoring undergraduate student summer research for underrepresented groups; (2) engaging high school female students in science and engineering; and (3) providing underrepresented/minority graduate students with unique research opportunities.Technical Summary:A major challenge in tissue regeneration is to design scaffolds that simultaneously induce endogenous regeneration of multiple tissues with inherently low regenerative potential. To address the challenge, this project will create scaffolds capable of not only recruiting different types of endogenous cells, but also guiding the recruited cells to concurrently regenerate into different tissues. The project will use fibrous scaffolds that mimic the morphology of tissue extracellular matrix, and periodontal cells as model cells to gain a fundamental understanding of the following relationships: (1) the effect of chemotactic growth factor release on the activation of chemotactic migration of multiple cell types; (2) how biological signals such as immobilized proteins direct the differentiation and matrix deposition of these cell types; and (3) how scaffold morphology can be tailored to act as a physical barrier to the cells that may delay the regeneration while inducing the desired cells to populate the scaffolds. Understanding these relationships will guide scaffold design for simultaneous regeneration of tissues. The proposed studies will foster education and outreach activities including mentoring the research of underrepresented undergraduate and graduate students, and engaging high school female students in science and engineering.

非技术综述：支架已广泛用于组织再生。然而，设计同时诱导多种组织内源性再生的支架仍然具有挑战性，这些组织具有固有的低再生潜力，如牙周组织和肌肉骨骼组织。目前的支架缺乏同时将不同类型的细胞招募到病变部位并促进它们发育成组织的能力。这个项目将设计脚手架来应对当前的挑战。支架将以牙周细胞为模型细胞进行测试。这些研究将从根本上了解支架的物理和生物学特性对几种细胞类型同时招募的影响，并指导它们再生成相应的组织。该项目的成果将指导多种组织结构的同时再生支架的开发。拟议的研究与多方面的教育和推广计划相结合。它包括(1)指导本科学生的暑期研究；(2)吸引高中女生参与科学和工程；以及(3)为代表不足的/少数族裔研究生提供独特的研究机会。技术概述：组织再生的一个主要挑战是设计支架，同时诱导多种组织的内源性再生，再生潜力天生较低。为了应对这一挑战，该项目将创造出不仅能够招募不同类型的内源性细胞，而且能够引导招募的细胞同时再生为不同组织的支架。该项目将使用模拟组织细胞外基质形态的纤维支架，并以牙周细胞为模型细胞，以获得对下列关系的基本了解：(1)趋化生长因子释放对激活多种类型细胞的趋化迁移的影响；(2)固定化蛋白质等生物信号如何指导这些类型的细胞的分化和基质沉积；以及(3)如何定制支架形态以充当细胞的物理屏障，可能延迟再生，同时诱导所需的细胞填充支架。了解这些关系将指导同时再生组织的支架设计。拟议的研究将促进教育和外联活动，包括指导代表性不足的本科生和研究生的研究，以及让高中女生参与科学和工程学。