Collaborative Research: Tunable aptasensors for analysis and sorting of living cells

合作研究：用于活细胞分析和分选的可调谐适体传感器

• 批准号: 1160262
• 负责人: Alexander Revzin
• 金额: $ 22.5万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-04-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1160262&HistoricalAwards=false
• 关键词: Collaborative; Research; Tunable; aptasensors; analysis

1160262/ RevzinThe Intellectual MeritThe goal of this proposal is to develop biosensors based on nucleic acids called aptamers. Aptamer based biosensors are emerging as viable alternative to traditional sensors that employ antibodies for recognition of target molecules. Aptamer based biosensors are more chemically and thermally stable and may be reagentless - that is no additional processing steps are needed to generate a signal in response to target molecules. Our proposal seeks to addresses a distinct challenge in regenerating these biosensors in order to make the same sensor available for continuous in-line monitoring. We propose to use applied voltage to tune and control affinity of sensing electrodes in temporal and/or spatial manner to ensure that some electrodes are detecting association while others are monitoring dissociation events. This way up and down changes in the level of target molecules may be monitored. Broader ImpactThe goal of our proposal will be to develop novel, aptamer-based microsystems for analysis and sorting of cells. It is our belief that such aptasensors are particularly advantageous for monitoring dynamic changes in the levels of proteins secreted by living cells and have numerous applications in biotechnology, cell/tissue engineering and infectious disease detection. In addition to biosensing, aptamer-modified surfaces may be used for affinity separation of cells from heterogeneous samples such as blood. This proposal will combine aspects of basic science with bioengineering to further enhance state of the art in cell analysis and manipulation by developing tunable aptamer-coated devices. We will actively recruit graduate, undergraduate and high school students to participate in the proposed experiments.

1160262/Revzin智力上的优点该提案的目标是开发基于核酸的生物传感器，称为适体。 基于适体的生物传感器正在成为采用抗体识别靶分子的传统传感器的可行替代方案。 基于适体的生物传感器在化学和热方面更稳定，并且可以是无试剂的，即不需要额外的处理步骤来产生响应于靶分子的信号。我们的提案旨在解决再生这些生物传感器的独特挑战，以便使相同的传感器可用于连续在线监测。 我们提出使用所施加的电压来以时间和/或空间方式调谐和控制感测电极的亲和力，以确保一些电极检测关联，而其他电极监测解离事件。 这样，可以监测靶分子水平的上下变化。 更广泛的影响我们提案的目标是开发新的，基于适体的微系统，用于细胞的分析和分选。 我们相信，这种适体传感器对于监测活细胞分泌的蛋白质水平的动态变化特别有利，并且在生物技术、细胞/组织工程和传染病检测中具有许多应用。 除了生物传感，适体修饰的表面可用于从异质样品如血液中亲和分离细胞。 该提案将联合收割机方面的基础科学与生物工程，以进一步提高国家的最先进的细胞分析和操作，通过开发可调适体涂层设备。 我们将积极招募研究生、本科生和高中生参加拟议的实验。