"PESO: Microfabricated surfaces for analysis of exosome-based paracrine signaling in hepatocellular carcinomas"

“PESO：用于分析肝细胞癌中基于外泌体的旁分泌信号传导的微加工表面”

• 批准号: 1233617
• 负责人: Alexander Revzin
• 金额: $ 54万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1233617&HistoricalAwards=false
• 关键词: PESO; Microfabricated; surfaces; analysis; exosome

1233617/ RevzinThis award is supported through a joint program between the National Science Foundation and the National Cancer Institute. Cancers originate in micrometere scale regions of tissues and organs called cancer microenvironment. This microenvironment consists of healthy as well as cancerous cells that are present in close proximity and signal to each other via soluble molecules. The study of cell signaling or communication is important as it may reveal why some cancers remain dormant while others become aggressive, metastasizing from the primary site. The goal of this grant is to investigate a new mechanism of intercellular communication in cancers. This mechanism is based on lipid particles called exosomes, secreted by cancer cells and carrying within them the payload of nucleic acids and proteins. The researchers hypothesize that uptake of exosomes by neighboring non-cancerous cells may facilitate cancer progression and metastasis. The goal of the work will be to develop novel sensing techonologies for detection of exosome production and novel cell culture approaches for assessing the impact of exosome-producing cancer cells on neighboring non-cancer cells. The research efforts will focus on the study of liver cancer.

该奖项由美国国家科学基金会和美国国家癌症研究所的联合项目资助。癌症起源于被称为癌症微环境的组织和器官的微米级区域。这种微环境由健康细胞和癌细胞组成，它们相互靠近，并通过可溶性分子相互传递信号。细胞信号或通讯的研究很重要，因为它可能揭示为什么一些癌症保持休眠状态，而另一些癌症变得具有攻击性，从原发部位转移。这项资助的目的是研究癌症细胞间通讯的新机制。这种机制是基于被称为外泌体的脂质颗粒，由癌细胞分泌，并在其中携带核酸和蛋白质的有效载荷。研究人员推测，邻近的非癌细胞摄取外泌体可能促进癌症的进展和转移。这项工作的目标将是开发新的检测外泌体产生的传感技术和新的细胞培养方法，以评估产生外泌体的癌细胞对邻近非癌细胞的影响。研究工作将集中在肝癌的研究上。