SBIR Phase I: Bio-finFET Based Portable Electronic Sensor to Quantify Contaminant Levels in Food and Dairy Products

该小型企业创新研究（SBIR）第一阶段项目旨在开发一种新的生物传感器平台，以同时检测食品和乳制品中的多种污染物（掺假化学品，抗生素和病原体）。生物传感器产品采用便携式配置，包括低成本的电子传感器条和手持式读取器设备。所提出的能够实现毫微微摩尔灵敏度的传感器技术基于正在申请专利的硅生物化学鳍式场效应晶体管（bio-finFET），类似于纳米线FET。该项目旨在建立这种技术，用于概念验证免疫测定测试，以超灵敏，快速，低成本和无标签定量各种食品污染物，如三聚氰胺，抗生素，如链霉素（选择用于概念验证），以及食源性病原体（如本研究中的沙门氏菌）。提出的Si bio-finFET芯片技术有可能成为一种通用的检测平台，可以将LFI（低成本、便携、快速检测）和ELISA（多分析物检测、特异性好）的优点联合收割机结合起来。此外，bio-finFET可以提供比ELISA或LFI更好的灵敏度，但无需分子标记或染色。该项目更广泛的影响/商业潜力源于食品安全检测的强劲需求和市场需求;具体地说，需要改进的用于测试各种食品安全危害的方法，这些方法可以提供高特异性、高准确性和污染物水平的定量、快速测试、低成本，和消费者以及供应链的可访问性，在单个测试程序中具有多分析物能力。该第一阶段提案的目标是实现所有这些目标的概念验证，并在各个方面提供比现有方法更优越的上级性能。到2015年，全球食品安全污染物检测市场预计将增长至41亿美元，复合年增长率为10.46%。近年来，奶制品中的三聚氰胺掺假一直是一个主要问题，主要是在中国，导致30万人患病，至少6人死亡。乳制品安全的其他主要问题包括抗生素残留和病原体-该第1阶段项目将在所有三个领域展示概念验证能力。乳制品检测是整个食品安全检测市场的一个重要和相当大的部分;由于牛奶和乳制品在健康和营养方面的重要性，以及所涉及产品的经济价值。

This Small Business Innovation Research (SBIR) Phase I project aims to develop a new biosensor platform to simultaneously detect multiple contaminants (adulteration chemicals, antibiotics, and pathogens) in food and dairy products. The biosensor products are in a portable configuration, consisting of low-cost, electronic sensor strips and hand-held reader devices. The proposed sensor technology capable of femtoMolar sensitivity is based on patent-pending Si biochemical-fin field effect transistor (bio-finFET), similar to nanowire FETs. The project aims to establish this technology for proof-of-concept immunoassay tests for ultrasensitive, rapid, low-cost, and label-free quantification of various food contaminants such as melamine, antibiotics such as streptomycin (selected for proof-of-concept), and food borne pathogens (such as staphylococcus, in this study). The proposed Si bio-finFET chip technology has the possibility to become a general detection platform that can combine the advantages of LFI (low cost, portable, rapid test) and ELISA (multiple-analyte test, good specificity). Moreover, the bio-finFET can provide much better sensitivity than ELISA or LFI but without molecular labeling or staining.The broader impact/commercial potential of this project stems from the strong demand and market needs for food safety testing; specifically, the need for improved methods for testing for various food-safety hazards that can provide high specificity, high accuracy and quantification of contaminant levels, rapid testing, low cost, and accessibility to consumers as well as to the supply chain, with multi-analyte capability in a single test procedure. This Phase 1 proposal is targeted at proof-of-concept in meeting all these objectives, and providing superior performance to existing methods in all regards. The global food safety testing market by contaminants is estimated to grow to $4.1B by 2015, growing at a CAGR of 10.46 %. Melamine adulteration of milk products has been a major issue in recent years, primarily in China, resulting in 300,000 illnesses and at least 6 deaths. Other major concerns with milk product safety include antibiotic residues, and pathogens - this Phase 1 project will demonstrate proof-of-concept capability in all three areas. Milk product testing represents an important and sizable segment of the overall food-safety testing market; due to the importance of milk and dairy products in health and nutrition, as well as the financial value of the products involved.