Acquisition of an Illumina Hi-Seq 2500

描述（由申请人提供）： 新一代高通量DNA测序仪的发展导致了生物医学研究的转变。这些仪器在全基因组规模上提供了广泛的应用，包括SNP发现、转录组分析、靶向外显子组测序和蛋白质-核酸相互作用的鉴定。该提案旨在通过增加Illumina HiSeqTM 2500系统下一代DNA测序仪来扩大罗切斯特大学功能基因组学中心（FGC）的高通量测序能力。FGC中现有的下一代功能由单个Illumina GAIIxTM仪器支持。用新的HiSeqTM 2500取代GAIIxTM将大大扩大FGC的容量，以满足我们两个校区（罗切斯特大学医学和牙科学院（SMD）和罗切斯特大学主校区（UR））对高通量测序服务日益增长的需求。由于其显著更大的通量和更快的运行时间，使用HiSeqTM 2500仪器将为研究人员节省研究项目的时间和成本。该仪器将主要由10个主要用户和8个次要用户组成的小组使用，这些用户是现有强大的Illumina用户群的成员，他们拥有多种NIH资助的项目。由于HiSeqTM 2500将取代FGC中现有的GAIIxTM，因此它将很容易集成到既定的工作流程中，而无需额外的辅助设备，员工或计算基础设施。FGC是现有多核心结构的一部分，为SMD研究人员提供共享资源，包括DNA测序，微阵列，蛋白质组学，代谢组学以及信息学和统计支持。FGC为高通量测序提供的支持包括初始咨询、样品制备、文库构建和测序。生物信息学和统计支持通过FGC由与综合研究计算中心（CIRC）和生物统计学和计算生物学系整合的服务提供。这些综合资源和支持将使经验丰富的研究人员以及不熟悉高通量测序的研究人员能够立即利用HiSeqTM 2500的功能。添加HiSeqTM 2500作为具有成本效益的共享资源将为多个NIH资助的项目提供实质性的好处，并刺激新研究人员开展更多项目，使他们能够开发未来的NIH资助项目，并在最高科学水平上竞争。

DESCRIPTION (provided by applicant): Development of a new generation of high-throughput DNA sequencers has led to a transformation of biomedical research. These instruments provide a wide range of applications on a whole genome scale, including SNP discovery, transcriptome profiling, targeted exome sequencing and identification of protein- nucleic acid interactions. This proposal seeks funds to expand high-throughput sequencing capacity of the University of Rochester Functional Genomics Center (FGC) by addition of an Illumina HiSeqTM 2500 System next-generation DNA sequencer. Existing next-generation capabilities within the FGC are supported by a single Illumina GAIIxTM instrument. Replacement of the GAIIxTM with the new HiSeqTM 2500 would substantially expand capacity of the FGC to meet the increasing demand for high-throughput sequencing services at our two campuses, the University of Rochester School of Medicine and Dentistry (SMD) and the University of Rochester main campus (UR). As a result of a significantly larger throughput and a faster run time, access to the HiSeqTM 2500 instrument will save investigators time and cost on their research projects. This instrument will primarily be used by a group of 10 Major and 8 Minor Users who are members of an existing strong Illumina user base with a diversity of NIH funded projects. Because the HiSeqTM 2500 will replace the existing GAIIxTM in the FGC, it will be easily integrated into an established workflow without the need for additional ancillary equipment, staff or computing infrastructure. The FGC is part of an existing multi-core structure providing shared resources to SMD investigators, including DNA sequencing, microarrays, proteomics, metabolomics as well as informatics and statistical support. Support provided by the FGC for high-throughput sequencing includes initial consultation, sample preparation, library construction and sequencing. Bioinformatics and statistical support is provided through the FGC by services integrated with the Center for Integrated Research Computing (CIRC) and The Department of Biostatistics and Computational Biology. These combined resources and support will allow experienced investigators as well as investigators unfamiliar with high-throughput sequencing to take immediate advantage of the HiSeqTM 2500 capabilities. Addition of the HiSeqTM 2500 as a cost effective shared resource will provide a substantial benefit to multiple NIH funded projects and stimulate additional projects among new investigators, enabling them develop future NIH funded projects and compete at the highest scientific level.