MRI: Acquisition of an Integrated Fluorescence and Atomic Force microscope (IF-AFM) for biophysics, biomaterials and nanomedicine studies

MRI：采购集成荧光和原子力显微镜 (IF-AFM)，用于生物物理学、生物材料和纳米医学研究

• 批准号: 1229284
• 负责人: Peter Hoffmann
• 金额: $ 34.55万
• 依托单位: Wayne State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2015-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1229284&HistoricalAwards=false
• 关键词: MRI; Acquisition; Integrated; Fluorescence; Atomic

This award to Wayne State University is for the acquisition of an integrated fluorescence and atomic force microscope to create a new platform to perform nanoscale measurements of cell-particle interactions. AFM is a force-measuring and surface imaging technique that can provide very high spatial resolution, while fluorescence microscopy provides fast, large scale and specific imaging of cells. By combining the two, (1) single molecule force measurements will be correlated with biological microenvironments or events, (2) simultaneous fluorescence and AFM imaging for a fuller picture of biological systems and their dynamics will be perform, and (3) sophisticated fluorescence techniques, such as Förster resonance energy transfer (FRET), can be correlated with AFM to measure applied forces to changes in structure. The combined instrumental platform will be used for interdisciplinary projects including single molecule interactions on live cells, optimization of DNA release from engineered polymer systems, cell motility studies, interactions of pathogens with cell surfaces, detection of food-borne bacteria, and dynamics and transport of magnetic nanoparticles in live cells.The new instrumental platform will be a user facility, available to researchers in physics, materials science, biomedical engineering, biology, chemistry, the basic medical sciences, and beyond. The collaborative use of this instrument is expected to foster interactions between different disciplines, and thus create new opportunities and ideas. The proposed research has clear potential to lead to new technologies and benefit human health. The new platform will be integrated into an advanced biophysics lab course, part of the Wayne State University undergraduate Biomedical Physics Program, which impacts a significant number of people from underrepresented groups. As a user facility, this new instrument will help to train and inspire future scientists and engineers.

这个奖项给韦恩州立大学是为了收购一个集成的荧光和原子力显微镜，以创建一个新的平台，执行纳米尺度的测量细胞-颗粒相互作用。 AFM是一种力测量和表面成像技术，可以提供非常高的空间分辨率，而荧光显微镜提供快速，大规模和特定的细胞成像。 通过结合两者，（1）单分子力测量将与生物微环境或事件相关，（2）同时进行荧光和AFM成像，以更全面地了解生物系统及其动力学，以及（3）复杂的荧光技术，如Förster共振能量转移（FRET），可以与AFM相关，以测量结构变化的作用力。 该组合仪器平台将用于跨学科项目，包括活细胞上的单分子相互作用，工程聚合物系统中DNA释放的优化，细胞运动性研究，病原体与细胞表面的相互作用，食源性细菌的检测以及活细胞中磁性纳米颗粒的动力学和运输。新的仪器平台将是一个用户设施，可供物理学，材料科学、生物医学工程、生物学、化学、基础医学等。 合作使用这一工具有望促进不同学科之间的互动，从而创造新的机会和想法。 拟议中的研究显然有可能导致新技术并有益于人类健康。 新平台将被整合到一个先进的生物物理实验室课程，韦恩州立大学本科生物医学物理课程的一部分，这影响了来自代表性不足群体的大量人员。 作为一个用户设施，这个新仪器将有助于培训和激励未来的科学家和工程师。