MRI: Acquisition of a Fluorescence Activated Cell Sorter (FACS)

MRI：获取荧光激活细胞分选仪 (FACS)

• 批准号: 1229309
• 负责人: Vassilios Sikavitsas
• 金额: $ 39.43万
• 依托单位: University of Oklahoma Norman Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-15 至 2015-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1229309&HistoricalAwards=false
• 关键词: MRI; Acquisition; Fluorescence; Activated; Cell

This proposal is for the acquisition of a state-of-the-art Fluorescence Activated Cell Sorter (FACS) that will become part of a flow cytometry/cell sorting shared use facility appealing to a diverse set of research programs on the University of Oklahoma (OU) campus in Norman. Cell sorting is recognized as important and indispensable tools in fields ranging from basic biology in the life sciences to translational biomedical engineering research. Acquisition of state-of-the-art cell sorting capabilities on the OU-Norman campus would not only make available needed resources, but would also provide a powerful complement to existing cellular characterization facilities on the OU-Norman Campus: Scanning Electron Microscopy, Multi-photon Laser Scanning Confocal Microscopy, and Atomic Force Microscopy. Combining detailed information about individual cell structures provided by these microscopy techniques with (i) the extensive statistical information about cell populations from high throughput flow cytometry and (ii) the ability to identify and sort cells from complex populations by immunophenotyping will foster new and innovative research directions across the OU campus.

该提案旨在购买最先进的荧光激活细胞分选仪 (FACS)，该设备将成为流式细胞术/细胞分选共享设施的一部分，吸引俄克拉荷马大学 (OU) 诺曼校区的各种研究项目。细胞分选被认为是从生命科学基础生物学到转化生物医学工程研究等领域重要且不可或缺的工具。在 OU-诺曼校区获得最先进的细胞分选能力不仅可以提供所需的资源，而且还可以为 OU-诺曼校区现有的细胞表征设施提供强大的补充：扫描电子显微镜、多光子激光扫描共焦显微镜和原子力显微镜。将这些显微镜技术提供的有关单个细胞结构的详细信息与（i）来自高通量流式细胞术的有关细胞群的广泛统计信息和（ii）通过免疫表型从复杂群体中识别和分选细胞的能力相结合，将在整个开放大学校园中培育新的和创新的研究方向。