Equipment: MRI: Track # 2 Development of a high-speed super-resolution stimulated Raman scattering (SRS) microscope

受激拉曼散射(SRS)显微镜是一种基于分子振动键的显示化学成分的非线性光学成像方法。SRS成像技术作为一种强大的光谱成像工具，在过去的十年里得到了迅速的发展，导致了生命科学的重大发现。最先进的(SOA)SRS仪器的空间分辨率约为300 nm，广域时间分辨率约为30赫兹，这受到光的绕射性质和相对较弱的拉曼散射信号的限制。加州大学圣地亚哥分校(UCSD)的一个具有互补专业知识的多学科团队将开发第一个超分辨率(SR)和高速SRS显微镜，这是一种超材料结构照明显微镜(MESIM)，具有5倍的分辨率增强和100倍的拉曼信号，因此与SOA相比速度有所提高。新仪器可能会在生命科学和生物医学领域带来新的发现，包括细胞代谢、神经科学、医学和微生物学。MESIM将设在加州大学圣迭戈分校的高通研究所，在颁奖期间和之后将在那里进行维护。该仪器将提供给加州大学洛杉矶分校和其他学术机构的教职员工，以及政府和行业合作者和用户。智能优点具有振动键灵敏度的超分辨率高速成像工具不仅对基础研究至关重要，可以可视化新结构，识别新现象，了解与生命相关的活动，并创造新的诊断方法，它还可以为研究新的疾病治疗配方提供巨大的好处。最重要的是，它可以作为开发和实践有效探测和监测具有分子特异性的纳米尺度动力学的新方法的平台，并可能演变为行业标准。更广泛的影响这项研究的更广泛的影响可能是广泛的。新的成像科学和技术扩展了知识边界，并可能通过提高生活质量来影响这个生物学时代。拟议中的MESIM的建设将服务于南加州的大片地区，拟议中的显微镜将使该地区和其他地理区域的用户受益。该项目还将在研究生和本科水平的科学和工程人力资源的教育和开发方面发挥重要作用，帮助培养这一多学科领域的未来科学家和工程师。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

Stimulated Raman scattering (SRS) microscopy is a nonlinear optical imaging method for visualizing chemical content based on molecular vibrational bonds. SRS imaging technique has been rapidly developed in the last decade as a powerful spectral imaging tool, leading to significant discoveries in the life sciences. The state-of-the-art (SOA) SRS instrument has a spatial resolution of about 300nm and wide-field temporal resolution of about 30Hz, which is limited by the diffraction nature of the light and the relatively weak Raman scattering signals. A multidisciplinary team at University of California, San Diego (UCSD) with complementary expertise will develop the first super-resolution (SR) and high-speed SRS microscope, a metamaterial-enabled structured illumination microscope (MESIM), with 5X resolution enhancement and 100X Raman signal thus giving speed enhancement compared to the SOA. The new instrument may lead to new discoveries in life sciences and biomedical fields, including cell metabolism, neuroscience, medicine, and microbiology. The MESIM will be located in the Qualcomm Institute at UCSD, where it will be maintained during and after the award period. The instrument will be available to faculty members from UCSD and other academic institutions, and government and industrial collaborators and users. Intellectual merit A super-resolution high-speed imaging tool with vibrational bond sensitivity is critical not only for fundamental research to visualize new structures, identify new phenomena, understand life relevant activities, and create new diagnosis methods, it can also provide tremendous benefit to investigations of new disease treatment recipes. Most importantly, it can serve as a platform for development and practice of new approaches for efficiently probing and monitoring dynamics in nanoscales with molecular specificity and could evolve into an industry standard. Broader ImpactsThe broader impacts of this research are potentially wide-ranging. New imaging science and technology extend the knowledge boundary and could impact this age of biology by improving the quality of life. Construction of the proposed MESIM will be serving a wide area of Southern California, and the proposed microscope will benefit users throughout this and other geographic areas. The project will also play a significant role in the education and development of human resources in science and engineering at the graduate and undergraduate levels, helping to train future scientists and engineers in this multidisciplinary field.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.