Molecular Sieving in Two-Dimensional Periodic Free-Energy Landscapes Created by Patterned Nanofluidic Devices

由图案化纳米流体装置创建的二维周期性自由能景观中的分子筛分

• 批准号: 1231826
• 负责人: Jianping Fu
• 金额: $ 36.04万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1231826&HistoricalAwards=false
• 关键词: Molecular; Sieving; Two; Dimensional; Periodic

PI: Fu, JianpingInstitution: University of Michigan - Ann Arbor Intellectual Merit: Development of efficient nanofluidic sieving structures represents a major step toward optimizing bioseparation methods and integrating them onto a fully integrated bioanalysis microsystem. In light of the current research thrust of nanofluidic proteomics, it becomes critically important to understand molecular transport properties in constraining nanofluidic sieving environments. Thus, the major goal of this research is to study, both experimentally and theoretically, molecular dynamics in confining nanofluidic environments and how such knowledge can be utilized to design novel nanofluidic sieving structures for advanced bioseparation. Different micro- and nano-fabrication methods will be applied to generate sub-100 nm nanofluidic sieving structures. These nanofluidic structures will be carefully examined to investigate how their separation performances (such as size selectivity and separation resolution) are affected by different structural parameters of the nanofluidic structures and the external electric fields. Experimental results will be used to guide developments of kinetic models and further validate them. Reciprocally, predictions from theoretical modeling will be used to motivate new separation assays and guide improved designs and generations of the nanofluidic sieving structures. The two-way validation process between experiments and theoretical modeling will lead to a rigorous understanding of molecular dynamics in the confining nanofluidic geometry.Broader Impacts: Owing to its cross-disciplinary nature, the proposed research will seamlessly integrate knowledge from distinct fields including micro/nanofabrication, micro/nanofluidics, hindered transport, polymer physics, and fluorescence imaging of single molecules. The proposed research, if successful, will foster transformative progress for separation of physiologically-relevant molecules using synthetic nanofluidic structures, a critical step toward fulfilling the promise of the nanofluidic proteomic research and future highly integrated bioanalysis micro/nanosystems. The proposed research will also lead to a thorough understanding of molecular dynamics in the confining nanofluidic geometry, which could provide a novel basis for ultra-sensitive and high-resolution sensors and medical diagnostic systems. The proposed educational activities will have broad impacts on students from different educational levels and genders and ethnicities. Some of the technologies described in the proposal will be used as vehicles for outreach activities to K-12 students and other underrepresented female and minority students in the Ann Arbor and Ypsilanti school districts. The proposed outreach activities will reveal to K-12 students the exciting challenges in science and engineering and their close relevance to our society, thus motivating them to pursue science and engineering curricula. For undergraduate and graduate education, an interdisciplinary course in Micro/Nanofluidics and BioMEMS will be developed. This course will prepare engineering students to pursue research in a variety of multidisciplinary areas such as BioMEMS and Lab-on- Chip, analytical chemistry, and micro/nanoscale materials sciences.

主要研究者：Fu，Jianping机构：密歇根大学-安阿伯学术成就：高效纳米流体筛分结构的开发代表了优化生物分离方法并将其集成到完全集成的生物分析微系统中的重要一步。鉴于目前的研究推力的纳米流体蛋白质组学，它变得至关重要的是要了解分子的运输性能，在约束纳米流体筛分环境。因此，本研究的主要目标是研究，实验和理论，分子动力学限制纳米流体环境，以及如何利用这些知识来设计新的纳米流体筛分结构先进的生物分离。不同的微米和纳米制造方法将被应用于产生亚100纳米的纳米流体筛分结构。这些纳米流体结构将被仔细检查，以研究它们的分离性能（如尺寸选择性和分离分辨率）是如何受到纳米流体结构的不同结构参数和外部电场的影响。实验结果将用于指导动力学模型的开发并进一步验证它们。反过来，从理论建模的预测将被用来激励新的分离测定和指导改进的设计和代的纳米流体筛分结构。实验和理论建模之间的双向验证过程将导致严格理解的分子动力学的限制nanofluidic geometrics.Broader的影响：由于其跨学科的性质，拟议的研究将无缝集成从不同领域的知识，包括微/纳米纤维，微/纳米流体，受阻运输，聚合物物理和荧光成像的单分子。拟议的研究如果成功，将促进使用合成纳米流体结构分离生理相关分子的变革性进展，这是实现纳米流体蛋白质组学研究和未来高度集成的生物分析微/纳米系统的关键一步。拟议的研究还将导致对限制性纳米流体几何结构中的分子动力学的透彻理解，这可以为超灵敏和高分辨率传感器和医疗诊断系统提供新的基础。拟议的教育活动将对不同教育水平、性别和种族的学生产生广泛影响。提案中描述的一些技术将被用作向安阿伯和伊普西兰蒂学区的K-12学生和其他代表性不足的女性和少数民族学生开展外联活动的工具。拟议的外展活动将向K-12学生揭示科学和工程方面令人兴奋的挑战及其与我们社会的密切相关性，从而激励他们追求科学和工程课程。对于本科生和研究生教育，将开发微/纳米流体和BioMEMS的跨学科课程。本课程将培养工程专业的学生从事各种多学科领域的研究，如BioMEMS和芯片实验室，分析化学和微/纳米材料科学。