Mixotrophic bacteria and the cryptic marine sulfur cycle: Mechanisms of carbon assimilation and sulfur oxidation in the Arctic96BD-19 GSO clade
混合营养细菌和神秘的海洋硫循环:北极96BD-19 GSO进化枝的碳同化和硫氧化机制
基本信息
- 批准号:1232840
- 负责人:
- 金额:$ 37.33万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2012
- 资助国家:美国
- 起止时间:2012-10-01 至 2015-09-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Intellectual merit: The ocean serves an immense reservoir of carbon, nitrogen, phosphorus, sulfur, and other elements required for all life. The active and diverse microbial populations that inhabit the oceans are responsible for mediating nutrient transformations that maintain the chemistry of seawater. A recent study identified a ubiquitous group of marine bacteria from the Arctic96BD-19 gamma-proteobacterial sulfur oxidizer (GSO) lineage that is closely related to known sulfur oxidizing species that fix inorganic carbon and oxidize sulfide in low-oxygen waters. The potential for GSOs to use reduced forms of sulfur in oxygenated waters suggests that they are a keystone species that link the marine carbon and sulfur cycles. The only known isolates from the Arctic96BD-19 lineage of GSOs are now in culture, allowing fundamental questions about their roles in carbon and sulfur cycling to be investigated. Preliminary data suggest that they use energy from the oxidation of sulfur to assimilate carbon. This project seek to address the overarching hypothesis that sulfur transformations provide the Arctic96BD- 19 lineage of GSOs with energy for organic and inorganic carbon cycling throughout the water column. Three specific hypotheses will be tested.1. Arctic96BD-19 cells assimilate either organic carbon or fixes inorganic carbon, depending on environmental conditions.2. Arctic96BD-19 cells oxidize thiosulfate via formation of a tetrathionate intermediate, or using the branched thiosulfate oxidation pathway.3. Arctic96BD-19 cells are ubiquitous sulfur oxidizers that assimilate organic and inorganic carbon through the Pacific Northwest.A combination of laboratory growth studies of the investigator's pure cultures and comparative genomic analyses will be used. The genomic data will be used to determine whether the Arctic96BD-19 cultures possess the genetic potential to oxidize reduced sulfur to sulfate (based on possession of known core and ancillary sulfur oxidation genes), which potential oxidation pathways are used, and whether they can fix inorganic carbon. These data will help guide the physiology studies by determining the most likely forms of inorganic and organic compounds that can be utilized.Broader impacts: Marine bacteria are critical players in global nutrient cycles, but many of their individual and community functions in the ecosystem are not well understood. Future oceanographers will need to use cultivation-dependent and cultivation-independent methods to identify metabolic process that shape microbial communities and impact biogeochemical cycles. A female graduate student and two undergraduate students will be trained to use molecular and physiological data to address hypotheses. Additionally, this project will increase national exposure of ocean sciences through digital films that introduce students (particularly ages 15 to 20) to unique and exciting aspects of oceanographic research. A cross-college collaboration between the UW College of the Environment and the College of Arts and Sciences will train Master of Communication in Digital Media students to produce short films that encourage interest in oceanography among young people. Student education, scientific advancement, and public awareness are all important components of this project.
智力优势:海洋是碳、氮、磷、硫和所有生命所需的其他元素的巨大储存库。栖息在海洋中的活跃而多样的微生物种群负责介导维持海水化学性质的营养转化。最近的一项研究确定了一个普遍存在的海洋细菌群,来自北极96 BD-19 γ-变形菌硫氧化剂(GSO)谱系,与已知的硫氧化物种密切相关,这些硫氧化物种在低氧沃茨中固定无机碳并氧化硫化物。地球静止轨道组织在含氧沃茨中使用还原态硫的潜力表明,它们是连接海洋碳和硫循环的关键物种。北极96 BD-19系GSO的唯一已知分离物现在正在培养中,允许对其在碳和硫循环中的作用进行研究。 初步数据表明,它们利用硫氧化产生的能量来吸收碳。该项目试图解决一个总体假设,即硫的转化为北极96 BD- 19系列地球静止轨道提供了能量,使有机碳和无机碳在整个水柱中循环。三个具体的假设将被测试。1.北极96 BD-19细胞吸收有机碳或固定无机碳,取决于环境条件。Arctic 96 BD-19细胞通过形成连四硫酸盐中间体或使用分支的硫代硫酸盐氧化途径氧化硫代硫酸盐。北极96 BD-19细胞是通过太平洋西北部同化有机和无机碳的普遍存在的硫氧化剂。将使用研究者纯培养物的实验室生长研究和比较基因组分析的组合。基因组数据将用于确定Arctic 96 BD-19培养物是否具有将还原硫氧化为硫酸盐的遗传潜力(基于拥有已知的核心和辅助硫氧化基因),使用哪些潜在的氧化途径,以及它们是否可以固定无机碳。更广泛的影响:海洋细菌是全球营养循环的关键参与者,但它们在生态系统中的许多个体和群落功能尚不清楚。未来的海洋学家将需要使用依赖培养和不依赖培养的方法来确定塑造微生物群落和影响生物地球化学循环的代谢过程。一名女研究生和两名本科生将接受培训,使用分子和生理数据来解决假设。此外,该项目将通过数字电影向学生(特别是15至20岁的学生)介绍海洋学研究的独特和令人兴奋的方面,增加国家对海洋科学的了解。UW环境学院和艺术与科学学院之间的跨学院合作将培养数字媒体学生的传播硕士,以制作短片,鼓励年轻人对海洋学的兴趣。学生教育、科学进步和公众意识都是这个项目的重要组成部分。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Robert Morris其他文献
Peer–to–Peer Overlays: Issues and Trends
点对点覆盖:问题和趋势
- DOI:
- 发表时间:
2013 - 期刊:
- 影响因子:0
- 作者:
Arockia Mary M. Radhakrishnan;E. Lua;J. Crowcroft;M. Pias;Ravi Sharma;Steven Lim;Timo Tanner;J. Buford;Heather Yu;Eng Keong Lua quotP2P;Karl Aberer;M. Hauswirth;Ion Stoica;Robert Morris;David Karger;M. Kaashoek;Hari Balakrishnan;Jessie Hui Wang;Chungang Wang;Jiahai Yang;Hiroshi Nishida;Thinh Nguyen;Murat Karakaya;I. Korpeoglu - 通讯作者:
I. Korpeoglu
Prevalence of targetable and potentially targetable alterations in metastatic/recurrent cervical cancer specimens in a large genomic dataset
在一个大型基因组数据集中转移性/复发性宫颈癌样本中可靶向和潜在可靶向改变的流行率
- DOI:
10.1016/j.ygyno.2024.07.337 - 发表时间:
2024-11-01 - 期刊:
- 影响因子:4.100
- 作者:
John Wallbillich;Sami Nassar;Michael Wilson;Larissa Mattei;Elizabeth Johns;Radhika Gogoi;Robert Morris;Ira Winer - 通讯作者:
Ira Winer
Disease site matters: Ovarian carcinosarcoma has far more genomic similarity to high-grade serous ovarian carcinoma than to uterine carcinosarcoma
- DOI:
10.1016/s0090-8258(21)00894-5 - 发表时间:
2021-08-01 - 期刊:
- 影响因子:
- 作者:
Christopher Walker;Erin Spencer;Gregory Dyson;Rouba Ali-Fehmi;Ira Winer;Michele Cote;Robert Morris;John Wallbillich - 通讯作者:
John Wallbillich
Double Opposing Zplasty with VY Advancement of the perineum: Long-term results of a new technique as an alternative to Fenton’s operation for narrowing and splitting of the skin at the posterior vaginal fourchette
- DOI:
10.1016/j.ejogrb.2018.02.003 - 发表时间:
2018-04-01 - 期刊:
- 影响因子:
- 作者:
Jonathan Frappell;Lia Riadin;Elhami Ebeid;Tilo Asmussen;Robert Morris - 通讯作者:
Robert Morris
The cost of convenience: Fellow and program director perspectives on the adequacy of virtual fellowship interviews as a replacement for in-person interviews (2317)
便利性的代价:研究员和项目主任对虚拟研究员面试作为面对面面试替代品的充分性的看法(2317)
- DOI:
10.1016/j.ygyno.2023.06.434 - 发表时间:
2023-09-01 - 期刊:
- 影响因子:4.100
- 作者:
Larissa Mattei;Madison Miller;Lauren Robb;Rosa Polan;Radhika Gogoi;Robert Morris - 通讯作者:
Robert Morris
Robert Morris的其他文献
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{{ truncateString('Robert Morris', 18)}}的其他基金
Collaborative Research: Drivers and effects of latent phage activation in marine SAR11
合作研究:海洋 SAR11 中潜在噬菌体激活的驱动因素和影响
- 批准号:
2201310 - 财政年份:2022
- 资助金额:
$ 37.33万 - 项目类别:
Standard Grant
CSR: Medium: Collaborative Research: Soup: Flexible Storage and Processing for On-Line Applications
CSR:媒介:协作研究:汤:在线应用程序的灵活存储和处理
- 批准号:
1704172 - 财政年份:2018
- 资助金额:
$ 37.33万 - 项目类别:
Continuing Grant
CSR: Small: Operating Systems Kernels in High-Level Languages
CSR:小:高级语言的操作系统内核
- 批准号:
1617487 - 财政年份:2016
- 资助金额:
$ 37.33万 - 项目类别:
Standard Grant
Characterizing the contribution of bacteria from the SUP05 clade to autotrophic and heterotrophic carbon cycling across ocean gradients
表征 SUP05 进化枝细菌对跨海洋梯度自养和异养碳循环的贡献
- 批准号:
1558483 - 财政年份:2016
- 资助金额:
$ 37.33万 - 项目类别:
Standard Grant
Program Development in Cybersecurity with Focus on Business and Healthcare Concepts
以商业和医疗保健概念为重点的网络安全项目开发
- 批准号:
1104106 - 财政年份:2011
- 资助金额:
$ 37.33万 - 项目类别:
Standard Grant
CSR-PSCE, SM: An Operating System for Multi-core Processors
CSR-PSCE、SM:多核处理器操作系统
- 批准号:
0834415 - 财政年份:2008
- 资助金额:
$ 37.33万 - 项目类别:
Continuing Grant
NeTS-FIND: User Information Architecture
NeTS-FIND:用户信息架构
- 批准号:
0627065 - 财政年份:2006
- 资助金额:
$ 37.33万 - 项目类别:
Standard Grant
SBIR Phase I: Tools for Information Retrieval and Document Classification Using Fast Phonetic Word-Spotting Technology
SBIR 第一阶段:使用快速语音单词识别技术的信息检索和文档分类工具
- 批准号:
0441492 - 财政年份:2005
- 资助金额:
$ 37.33万 - 项目类别:
Standard Grant
SEI(BIO)+II: Metadata-rich Image Databases for Biodiversity Applications
SEI(BIO) II:用于生物多样性应用的元数据丰富的图像数据库
- 批准号:
0430769 - 财政年份:2004
- 资助金额:
$ 37.33万 - 项目类别:
Standard Grant
SBIR Phase I: Collaborative Product Definition Management
SBIR 第一阶段:协作产品定义管理
- 批准号:
0339708 - 财政年份:2004
- 资助金额:
$ 37.33万 - 项目类别:
Standard Grant
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Cryptic gut bacterial metabolites that regulate colorectal cancer formation
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通过隐性翻译生成抗原肽/MHC
- 批准号:
7195935 - 财政年份:2000
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- 批准号:
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