RUI: Genetic Analysis of Gurken translational Control in Drosophila

RUI:果蝇 Gurken 翻译控制的遗传分析

基本信息

  • 批准号:
    1243951
  • 负责人:
  • 金额:
    $ 25.07万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    2013
  • 资助国家:
    美国
  • 起止时间:
    2013-02-01 至 2018-01-31
  • 项目状态:
    已结题

项目摘要

Intellectual merit: All animals must reproducibly establish their body plan in the face of a constantly changing environment. Gurken is an evolutionarily conserved growth factor that is carefully regulated to establish the anterior / posterior and dorsal / ventral axis during oogenesis in the fruit fly Drosophila melanogaster. This project aims to identify the mechanisms by which the translation of Gurken is regulated by addressing the response of gurken mRNA to two stimuli: Nutrient limitation and DNA-damage. The first goal of the research is to understand how Drosophila maintain robust developmental patterning when confronted with an environment where the availability of nutrients is highly variable. The production of proteins is highly regulated in response to nutrient availability and ensures the efficient utilization of resources. Translation in the cell is globally repressed when nutrients are scarce; however, a small subset of essential proteins escape this repression by employing an alternative mechanism known as IRES-mediated translation. Preliminary work from the PI's lab suggests that the gurken mRNA utilizes this alternative mechanism to maintain the axis patterning when nutrients are limiting. The research will utilize a number of genetic and biochemical approaches to study the activity of the gurken mRNA both in vitro and in vivo. Specifically, the activity of regulatory regions of the gurken mRNA will be probed by joining them to light emitting luciferase reporter genes. This will facilitate the discovery of IRES elements that allow the oocyte to maintain its robust patterning. The second goal of the research is to understand how damage to the genome that occurs during meiosis affects the translation of gurken. Previous research has shown that mutations that interfere with DNA repair inhibit the translation of gurken by an unknown mechanism. Several novel mutations that affect this signaling pathway have been isolated. This project will take a genomic sequencing approach to identify these mutations and shed light on the mechanism(s) that communicates the integrity of the genome to developmental patterning molecules.Broader Impact: The research conducted in this project will engage students in the study of translation regulation at the State University of New York College at Fredonia, a predominantly undergraduate institution. The research will address several pressing questions in the field, as indicated above, while allowing students to be the driving force behind the activities. The students will be intimately involved in the design and execution of the experiments and have the opportunity to present the results of the project at regional and national meetings. The research will directly impact not only the students working in the PI's laboratory, but also students that are enrolled in the core genetics lab and upper level molecular genetics lab courses (approximately 70 students per year). The project will facilitate full-time research experiences for three summer students that will be recruited from Fredonia and/or regional community colleges.
智力优势:面对不断变化的环境,所有动物都必须建立可复制的身体计划。Gurken是一种进化上保守的生长因子,在果蝇卵发生过程中被精心调节以建立前/后和背/腹轴。本项目旨在通过解决Gurken mRNA对两种刺激(营养限制和dna损伤)的反应来确定Gurken翻译受到调节的机制。这项研究的第一个目标是了解果蝇在面对营养物质高度可变的环境时是如何保持强健的发育模式的。蛋白质的生产受到高度调节,以响应营养的可用性,并确保资源的有效利用。当营养缺乏时,细胞内的翻译会受到全面抑制;然而,一小部分必需蛋白通过采用一种称为ires介导的翻译的替代机制来逃避这种抑制。PI实验室的初步工作表明,gurken mRNA利用这种替代机制在营养有限时维持轴的模式。该研究将利用一些遗传和生化方法来研究gurken mRNA在体外和体内的活性。具体来说,gurken mRNA的调控区域的活性将通过将其与发光荧光素酶报告基因连接来探测。这将有助于发现允许卵母细胞保持其强健模式的IRES元素。研究的第二个目标是了解在减数分裂期间发生的基因组损伤如何影响gurken的翻译。先前的研究表明,干扰DNA修复的突变通过一种未知的机制抑制了gurken的翻译。一些影响这一信号通路的新突变已经被分离出来。该项目将采用基因组测序方法来识别这些突变,并阐明将基因组完整性传达给发育模式分子的机制。更广泛的影响:在这个项目中进行的研究将吸引学生在纽约州立大学弗里多尼亚学院学习翻译法规,这是一个以本科生为主的机构。如上所述,该研究将解决该领域的几个紧迫问题,同时允许学生成为活动背后的驱动力。学生将密切参与实验的设计和执行,并有机会在区域和国家会议上展示项目成果。这项研究不仅会直接影响到在PI实验室工作的学生,还会影响到参加核心遗传学实验室和高级分子遗传学实验室课程的学生(每年约70名学生)。该项目将为三名来自弗里多尼亚和/或地区社区学院的暑期学生提供全职研究经验。

项目成果

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Scott Ferguson其他文献

THE MULTI-STATE BRONCHOSCOPY EDUCATION PROJECT: ACQUISITION OF COGNITIVE SKILLS
  • DOI:
    10.1378/chest.132.4_meetingabstracts.664
  • 发表时间:
    2007-10-01
  • 期刊:
  • 影响因子:
  • 作者:
    Gordon H. Downie;Mark Bowling;Gerard Silvestri;Scott Ferguson;Coakley Ray;John Conforti;Momen Wahidi
  • 通讯作者:
    Momen Wahidi
Bathymetric atlas and website for the Northwestern Hawaiian Islands
西北夏威夷群岛的测深地图集和网站
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    J. Miller;S. Vogt;R. Hoeke;Scott Ferguson;B. Applegate;J. R. Smith;M. Parke
  • 通讯作者:
    M. Parke
Addition of a telestrator for teaching during video-based procedures
  • DOI:
    10.1016/j.otohns.2009.12.043
  • 发表时间:
    2010-07-01
  • 期刊:
  • 影响因子:
  • 作者:
    Scott Ferguson;Brendan C. Stack
  • 通讯作者:
    Brendan C. Stack
Contribution of brain pericytes to neuroinflammation following repetitive head trauma
  • DOI:
    10.1186/s12950-025-00439-9
  • 发表时间:
    2025-03-03
  • 期刊:
  • 影响因子:
    4.100
  • 作者:
    Arianna Cembran;Maxwell Eisenbaum;Daniel Paris;Michael Mullan;Fiona Crawford;Scott Ferguson;Corbin Bachmeier
  • 通讯作者:
    Corbin Bachmeier
Deep learning-based real-time monitoring of electron beam powder bed fusion (EB-PBF) via electron emission
  • DOI:
    10.1007/s10845-025-02598-1
  • 发表时间:
    2025-04-17
  • 期刊:
  • 影响因子:
    7.400
  • 作者:
    Temilola Gbadamosi-Adeniyi;Scott Ferguson;Tim Horn
  • 通讯作者:
    Tim Horn

Scott Ferguson的其他文献

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{{ truncateString('Scott Ferguson', 18)}}的其他基金

Collaborative Research: Research: Investigating Jonassen’s Design Theory of Problem Solving in Support of Pedagogical Change in Introductory Aerospace Engineering
合作研究:研究:调查乔纳森的问题解决设计理论以支持航空航天工程入门教学的变革
  • 批准号:
    2118077
  • 财政年份:
    2021
  • 资助金额:
    $ 25.07万
  • 项目类别:
    Standard Grant
Workshop: NSF CAREER Proposal Writing Workshop for Early Career Professionals; Charlotte, North Carolina; August 23, 2016
研讨会:针对早期职业专业人员的 NSF 职业提案写作研讨会;
  • 批准号:
    1642438
  • 财政年份:
    2016
  • 资助金额:
    $ 25.07万
  • 项目类别:
    Standard Grant
Collaborative Research: Mitigating Emergent System Behavior through System Evolvability
协作研究:通过系统可进化性缓解紧急系统行为
  • 批准号:
    1301017
  • 财政年份:
    2013
  • 资助金额:
    $ 25.07万
  • 项目类别:
    Standard Grant
CAREER: Giving You "Almost" What You Want - Customizing Products Through the Integration of Market Modeling and Engineering Design
职业:给你“几乎”你想要的——通过市场建模和工程设计的整合定制产品
  • 批准号:
    1054208
  • 财政年份:
    2011
  • 资助金额:
    $ 25.07万
  • 项目类别:
    Standard Grant
Building Design Apps for Early Engineering Education
为早期工程教育构建设计应用程序
  • 批准号:
    1037677
  • 财政年份:
    2011
  • 资助金额:
    $ 25.07万
  • 项目类别:
    Standard Grant
GOALI: A Multidisciplinary Approach to Simultaneous Market Segmentation and Product Family Definition
GOALI:同时进行市场细分和产品系列定义的多学科方法
  • 批准号:
    0969961
  • 财政年份:
    2010
  • 资助金额:
    $ 25.07万
  • 项目类别:
    Standard Grant
Computer-based Equipment Platforms in Introductory Labs for Technology Students
技术学生入门实验室中基于计算机的设备平台
  • 批准号:
    9452536
  • 财政年份:
    1994
  • 资助金额:
    $ 25.07万
  • 项目类别:
    Standard Grant

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RUI:果蝇生殖细胞规范所需的保守分子支架 Tudor 复合物的生化和遗传分析
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  • 批准号:
    0640483
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  • 资助金额:
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RUI: Genetic and Molecular Analysis of Photoperiod Flowering in Arabidopsis
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