Chromosome evolution and genome stability: SeqA, TnsE and Transposon Tn7

染色体进化和基因组稳定性：SeqA、TnsE 和转座子 Tn7

• 批准号: 1244227
• 负责人: Joseph Peters
• 金额: $ 30.02万
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-02-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1244227&HistoricalAwards=false
• 关键词: Chromosome; evolution; genome; stability; SeqA

This research will explore the role of the bacterial protein SeqA in genome stability. This important but poorly understood protein appears to be involved in protecting cellular DNA during replication from deleterious recombination events. Themes established here can be extrapolated to other organisms and potentially lead to the development of genome manipulation procedures in important and diverse bacterial systems. This work will also help explain how a large and important family of mobile DNA elements or "jumping" genes that can move between sites in the DNA are capable of disseminating genetic information across vastly different types of bacteria using a protein called TnsE. This work also indicates features that are conserved across the most distantly related organisms on earth while highlighting a feature that allows a parasite to adapt to specific types of bacteria.Broader Impacts: The research will be disseminated widely at multiple venues including national and regional meetings. Tn7 is an important tool for modifying the genetic content of a broad variety of bacteria for basic science and industrial applications. The work will continue to facilitate such efforts. In addition to training a graduate student, the work will also involve multiple undergraduates in meaningful research experiences.

这项研究将探索细菌蛋白SeqA在基因组稳定性中的作用。 这种重要但知之甚少的蛋白质似乎在复制过程中保护细胞DNA免受有害重组事件的影响。这里建立的主题可以外推到其他生物体，并可能导致在重要和不同的细菌系统的基因组操作程序的发展。 这项工作还将有助于解释一个庞大而重要的移动的DNA元件或“跳跃”基因家族如何能够使用一种名为TnsE的蛋白质在不同类型的细菌中传播遗传信息。 这项工作还指出了地球上最远的生物体中保守的特征，同时突出了允许寄生虫适应特定类型细菌的特征。更广泛的影响：该研究将在包括国家和地区会议在内的多个场所广泛传播。Tn7是一种重要的工具，用于修改基础科学和工业应用中各种细菌的遗传内容。 这项工作将继续促进这些努力。 除了培养一名研究生，这项工作还将涉及多名本科生有意义的研究经验。