Chromosome scale genome assemblies for Culex pipiens mosquitoes

项目总结 来自CX的蚊子。复合型蚊虫是淋巴丝虫病和脑炎的主要传播媒介 病毒，包括西尼罗河病毒，它现在是美国最常见的虫媒病毒病。为了方便 开发先进的基于基因组的病媒控制策略，高质量的基因组组装， 基于第三代测序技术，为冈比亚按蚊物种开发了 复合体，以及埃及伊蚊和白纹伊蚊。然而，CX的基因组组装。比目鱼， 复合体中的模式物种仍然下落不明。我们的项目将开发高质量的基因组组件 该复合体的两名成员，CX。Popiens和Cx.P。猥亵。生态、行为和生理 亲缘关系密切的类群之间的差异往往与染色体倒置有关。然而，很少有人 库蚊的染色体倒置是由于其多丝质较差所致。 染色体。我们的项目将测试是否有任何染色体重排是分类群特有的或 与CX的局部适应有关。将蚊子传播到环境中。本项目的具体目标是：1) 为CX开发高质量的基因组组件。Popiens和Cx.P.molestus；2)创造物理 中国血吸虫基因组图谱。Popiens和Cx.以及3)染色体的鉴定 CX中的重排。Popiens和Cx.P。猥亵。我们将利用尖端的基因组 牛津纳米孔技术测序、生物纳米光学测绘、Hi-C支架和 基于cDNA的物理作图。将使用Hi-C方法和荧光原位杂交来 识别CX中的染色体倒位。Popiens和Cx.P。猥亵。我们展望了高科技的发展。 CX的两个成员的高质量基因组组装。及其复合体的鉴定 染色体重排，将进一步刺激病媒控制新策略的发展。

PROJECT SUMMARY Mosquitoes from the Cx. pipiens complex are principal vectors of lymphatic filariasis worms and encephalitis viruses, including West Nile virus, which is now the most common arboviral disease in the USA. To facilitate the development of advanced genome-based strategies for vector control, high-quality genome assemblies, based on third-generation sequencing technologies, were developed for species of the Anopheles gambiae complex, as well as for Aedes aegypti and Aedes albopictus. However, the genome assembly for Cx. pipiens, the type species in the complex, is still missing. Our project will develop high-quality genome assemblies for two members of the complex, Cx. p. pipiens and Cx. p. molestus. Ecological, behavioral, and physiological differences between closely related taxa are often associated with chromosomal inversions. However, very few chromosomal inversions have been described in Culex mosquitoes due to the inferior quality of their polytene chromosomes. Our project will test whether any of the chromosomal rearrangements are taxa-specific or related to local adaptation of Cx. pipiens mosquitoes to the environment. Specific aims for this project are to: 1) develop high-quality genome assemblies for Cx. p. pipiens and Cx. p. molestus; 2) create physical maps of the genomes of Cx. p. pipiens and Cx. p. molestus; and 3) identify chromosomal rearrangements in Cx. p. pipiens and Cx. p. molestus. We will utilize the cutting-edge genomic technologies of Oxford Nanopore Technology sequencing, BioNano optical mapping, Hi-C scaffolding, and cDNA-based physical mapping. The Hi-C approach and fluorescence in situ hybridization will be used to identify chromosomal inversions in Cx. p. pipiens and Cx. p. molestus. We envision that development of high- quality genome assemblies for two members of the Cx. pipiens complex, and identification of their chromosomal rearrangements, will further stimulate the development of new strategies for vector control.