Defining the Mechanistic Strategies of Defulfonation in the Alkanesulfonate Sulfonate Monooxygenase System

定义烷磺酸磺酸单加氧酶系统中去氟酰化的机制策略

基本信息

批准号：
1244320
负责人：
Holly Ellis
金额：
$ 67.72万
依托单位：
Auburn University
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2013
资助国家：
美国
起止时间：
2013-09-01 至 2018-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1244320&HistoricalAwards=false
关键词：
Defining Mechanistic Strategies Defulfonation Alkanesulfonate

项目摘要

Sulfur is an essential element for all life. Bacteria play a key role in the sulfur cycle through diverse metabolic pathways. When sulfur is limited in the environment, bacteria express specific proteins to retain intracellular sulfur. The alkanesulfonate monooxygenase system provides bacteria access to an alternative sulfur source under sulfur limiting conditions. These studies will elucidate the essential role of the alkanesulfonate monooxygenase system in supplying sulfur to bacteria. The broad objective of this project is to fully establish the mechanism used by the alkanesulfonate monooxygenase system to better understand its physiological role in sulfur acquisition and the overall sulfur cycle. An educational component of this project seeks to encourage students from underrepresented groups in the sciences to participate in research at Auburn University. The PI is collaborating with the Minority Programs Coordinator in the College of Science and Mathematics (COSAM) to participate in the Summer Bridge Program for incoming minority freshmen planning to major in sciences, mathematics, or pre-professional programs. An interactive talk on the importance of research in an undergraduate education will be presented followed by a hands-on laboratory experiment to stimulate interest in undergraduate research. The grant will provide a summer research fellowship to a minority student in COSAM for each funding year.

硫是所有生命的基本要素。通过各种代谢途径，细菌在硫周期中起关键作用。当硫在环境中受到限制时，细菌表达特定的蛋白质以保留细胞内硫。在硫限制条件下，烷烃单加氧化酶系统可提供对替代硫源的细菌。这些研究将阐明烷硫酸盐单加掺合酶系统在向细菌供应硫中的重要作用。该项目的广泛目的是充分建立烷硫酸盐单加氧化酶系统使用的机制，以更好地了解其在硫收采集和整体硫周期中的生理作用。该项目的一个教育部分旨在鼓励科学中代表性不足的群体的学生参加奥本大学的研究。 PI正在与科学与数学学院（COSAM）的少数群体计划协调员合作，参加了夏季桥梁计划，针对即将到来的少数族裔新生计划，以专业的科学，数学或专业课程。关于研究在本科教育中的重要性的互动演讲将进行，然后进行动手实验室实验，以刺激对本科研究的兴趣。该赠款将在每个资金一年为COSAM的少数族裔学生提供夏季研究奖学金。