Collaborative Research: BIOMAPS Control of Spindle Positioning and Cytokinesis

合作研究：BIOMAPS 控制纺锤体定位和细胞分裂

• 批准号: 1244441
• 负责人: Fred Chang
• 金额: $ 72.38万
• 依托单位: Columbia University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-01 至 2016-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1244441&HistoricalAwards=false
• 关键词: Collaborative; Research; BIOMAPS; Control; Spindle

Intellectual Merit: Cells typically divide at a specific, predictable location. Positioning of the cell division plane in cytokinesis is a fundamental and universal cellular process that has broad relevance to cell morphogenesis, development, tissue architecture, and stem cells. The aims of this research are to determine the molecular mechanisms governing this process. This project utilizes the sea urchin embryo as a potent animal cell model for symmetric cell division. Cell division site determination involves multiple processes. The cell initially positions and orients the nucleus in late interphase along a reproducible axis relative to its cell shape. In this process, the nucleus must somehow sense the shape of the cell. The axis of the interphase nucleus sets the position of the spindle in mitosis, which then specifies the site of cleavage in cytokinesis. The project will focus on the mechanisms of how the nucleus is positioned, how cell shape is sensed, and how the spindle signals to the cortex to specify the division plane. The researchers have recently developed a powerful new approach to study the effect of cell geometry in a systematic manner by manipulating the shape of sea urchin cells by introducing them into micro-fabricated chambers of different shapes (such as rectangles, triangles, etc. The project will include live cell experiments focusing on the identifying the location of and specific molecular motor responsible for nuclear positioning coupled with computational modeling of how the division plane is determined in cells manipulated into different shapes.Broader Impacts: The researchers continue longstanding efforts to increase the diversity of scientists by broadening participation of underrepresented minorities in science. Efforts will be directed both in the laboratory and in national activities. One of the PI's on this collaborative project is a cell biologist who is also a Native American (Cherokee) who has a long record of involvement in working with a diverse pool of students and in national programmatic efforts. The researchers share a laboratory at the MBL, Woods Hole for about two months each summer where they host undergraduates (mostly from underrepresented groups) recruited nationally at the Society for Advancement of Chicanos and Native Americans in Science (SACNAS) annual conference, from Boston College, and from the NSF funded REU summer program at the MBL. These students are mentored to participate in a number of activities whose aim is to better equip them, should they choose to pursue a scientific career. At the national level, the PI is continuing in a leadership role in programs whose goal is to increase the diversity of scientists including leading NSF supported efforts for SACNAS, for the American Society for Cell Biology and serving in a regular capacity to effect policy change, evaluate programs, and contribute to national activities by service to the AAAS, the NSF, and to colleges, universities and scientific societies.

智力优势：细胞通常在特定的、可预测的位置分裂。 胞质分裂中细胞分裂平面的定位是一个基本和普遍的细胞过程，与细胞形态发生、发育、组织结构和干细胞具有广泛的相关性。本研究的目的是确定控制这一过程的分子机制。 该项目利用海胆胚胎作为对称细胞分裂的有效动物细胞模型。 细胞分裂位点的确定涉及多个过程。细胞最初的位置和方向的细胞核在后期间期沿着一个可重复的轴相对于其细胞形状。 在这个过程中，细胞核必须以某种方式感知细胞的形状。 间期核的轴确定了有丝分裂中纺锤体的位置，从而确定了胞质分裂的位点。 该项目将集中在细胞核如何定位，细胞形状如何感知，以及纺锤体如何向皮质发出信号以指定分裂平面的机制。 研究人员最近开发了一种强大的新方法，通过将海胆细胞引入不同形状（如矩形，三角形等）的微制造室来操纵海胆细胞的形状，从而系统地研究细胞几何形状的影响。 该项目将包括活细胞实验，重点是确定负责核定位的特定分子马达的位置，以及如何在被操纵成不同形状的细胞中确定分裂平面的计算建模。更广泛的影响：研究人员继续长期努力，通过扩大在科学中代表性不足的少数群体的参与来增加科学家的多样性。 将在实验室和国家活动两方面作出努力。 这个合作项目的PI之一是一位细胞生物学家，他也是一位美洲原住民（切罗基人），他长期参与与不同学生的合作和国家计划工作。 研究人员在MBL共享一个实验室，伍兹霍尔每年夏天约两个月，他们在那里接待本科生（主要来自代表性不足的群体）在全国范围内招募在科学促进奇卡诺斯和美洲原住民协会（SACNAS）年度会议上，从波士顿学院，并从NSF资助的REU夏季计划在MBL。 指导这些学生参加一些活动，其目的是更好地装备他们，如果他们选择追求科学事业。在国家一级，PI继续在项目中发挥领导作用，其目标是增加科学家的多样性，包括为SACNAS，美国细胞生物学学会领导NSF支持的工作，并定期为AAAS，NSF以及学院，大学和科学学会服务，以影响政策变化，评估项目并为国家活动做出贡献。