Meeting: Workshop on Comparative Proteomics of Environmental and Pollution Stress, Cal Poly; December10-14, 2012

会议：环境和污染压力比较蛋白质组学研讨会，加州理工学院；

• 批准号: 1253059
• 负责人: Lars Tomanek
• 金额: $ 3.5万
• 依托单位: California Polytechnic State University Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-15 至 2013-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1253059&HistoricalAwards=false
• 关键词: Meeting; Workshop; Comparative; Proteomics; Environmental

The proteome of a cell is made up of the set of proteins being expressed by the cell at any one point in time. Proteins are the essential elements of a cell that enable nearly all of its functional capabilities, from providing the architectural elements that give the cell structure, facilitating cellular construction, regulating cellular metabolism and energy production, to cellular communication. The quantification of their synthesis patterns is critical to characterizing the response of an organism to a changing environment. Proteomics is the process of studying the dynamics of the proteome. While some studies have demonstrated much potential in the use of proteomics to improve understanding the response of organisms to potential climate change, few laboratories have ventured deeply into the study of the proteome, especially in the context of environmental stress. This is in large part due to the complexity and cost of the sophisticated analytical instrumentation required to separate, quantify and identify proteins. In addition, relatively few proteomics analyses exist of non-model organisms for which there is limited genomic information, despite the fact that they are often "model" systems in other biological disciplines, e.g., blue mussels (genus: Mytilus) as bio-indicators for pollution stress. This five day workshop will introduce twelve participants to the methodology involved in a proteomic analysis: from the separation of proteins by their charge (isoelectric point) and their molecular mass by two-dimensional gel electrophoresis and high-pressure liquid chromatography, the quantification of proteins and subsequent statistical analyses, to the identification of proteins through mass spectrometry, including matrix-assisted laser desorption tandem time of flight (MALDI-ToF-ToF) and electrospray ionization quadrupole time-of-flight (ESI Q-ToF) mass spectrometry. Importantly, participants will have the opportunity to analyze their own samples, generate their own data set, and will be exposed to the various tools that facilitate the interpretation of their results. The goal of this workshop is to introduce participants to proteomics methodologies and incubate several new proteomics projects.The PI has a track record of training a great number of undergraduates, graduate students, and visiting scholars in proteomic techniques, including mass spectrometry. He has collaborated with a number of colleagues to build a community around environmental proteomics, and organized a symposium on the topic at a recent conference. This workshop is a continuation of his effort to build a larger research community around environmental proteomics. Efforts will be made to recruit participants nationally. These efforts include a number of communications specifically directed to recruit participants from underrepresented groups in STEM, and therefore this project aims to broaden participation in these state-of-the-art approaches. Overall, the proposed workshop promises to build the capacity of the community to conduct proteomic analyses and will help initiate a number of new proteomic projects.

细胞的蛋白质组是由细胞在任何时间点上表达的一组蛋白质组成的。蛋白质是细胞的基本元素，几乎可以实现细胞所有的功能，从提供细胞结构的建筑元素，促进细胞构建，调节细胞代谢和能量产生，到细胞通讯。它们的合成模式的量化是表征生物体对不断变化的环境的反应的关键。蛋白质组学是研究蛋白质组动力学的过程。虽然一些研究已经证明了利用蛋白质组学来提高对生物对潜在气候变化的反应的理解的巨大潜力，但很少有实验室冒险深入研究蛋白质组学，特别是在环境胁迫的背景下。这在很大程度上是由于分离、量化和鉴定蛋白质所需的精密分析仪器的复杂性和成本。此外，对基因组信息有限的非模式生物进行的蛋白质组学分析相对较少，尽管它们通常是其他生物学学科中的“模式”系统，例如，蓝贻贝（Mytilus属）作为污染胁迫的生物指标。这个为期五天的研讨会将向十二位参与者介绍蛋白质组学分析的方法：从二维凝胶电泳和高压液相色谱通过电荷（等电点）和分子质量分离蛋白质，蛋白质的定量和随后的统计分析，到通过质谱法鉴定蛋白质，包括基质辅助激光解吸串联飞行时间（MALDI-ToF-ToF）和电喷雾电离四极杆飞行时间（ESI Q-ToF）质谱法。重要的是，参与者将有机会分析他们自己的样本，生成他们自己的数据集，并将接触到有助于解释其结果的各种工具。本次研讨会的目的是向参与者介绍蛋白质组学方法，并孵化几个新的蛋白质组学项目。PI在蛋白质组学技术（包括质谱）方面培养了大量本科生、研究生和访问学者。他与许多同事合作建立了一个围绕环境蛋白质组学的社区，并在最近的一次会议上组织了一个关于该主题的研讨会。这次研讨会是他围绕环境蛋白质组学建立一个更大的研究社区的努力的延续。将努力在全国范围内招募参与者。这些努力包括一些专门针对招募STEM中代表性不足群体的参与者的沟通，因此该项目旨在扩大这些最先进方法的参与。总的来说，提议的研讨会承诺建立社区进行蛋白质组学分析的能力，并将有助于启动一些新的蛋白质组学项目。