CAREER: The Function, Regulation, and Molecular Identity of Mechanosensitive Ion Channels in Arabidopsis

职业：拟南芥机械敏感离子通道的功能、调节和分子特性

• 批准号: 1253103
• 负责人: Elizabeth Haswell
• 金额: $ 133.04万
• 依托单位: Washington University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1253103&HistoricalAwards=false
• 关键词: CAREER; Function; Regulation; Molecular; Identity

INTELLECTUAL MERITIn land plants, many cellular and developmental processes necessitate the perception of mechanical stimuli such as touch, gravity, or osmotic pressure. One way in which mechanoperception is accomplished is through the activation of mechanosensitive channels, which allow ions to pass across a membrane in response to membrane stretch. This project aims to use the model flowering plant Arabidopsis thaliana to investigate the role played by established mechanosensitive channels during the process of fertilization and to determine how their activity is regulated. In addition, new plant mechanosensitive channels will be identified for future functional and comparative studies. This research will reveal the molecular mechanisms by which plants sense and respond to mechanical stimuli, provide insight into the evolution of mechanosensitive channels, and establish principles of mechanobiology that apply to other systems.BROADER IMPACTSAn inquiry-based laboratory class in ion channel physiology and two uniquely designed summer undergraduate research/outreach experiences will be developed, giving undergraduates an authentic research experience while contributing to the research goals of this project. Other activities will broaden the participation of underrepresented groups and enhance scientific understanding through the established outreach efforts surrounding the St. Louis Science Center's new ozone garden and Earth Day celebration. A running theme through all these activities is training in effective science communication. In addition, this research will benefit society by addressing the mechanisms by which plant growth, development, fertility, health, and response to stress. The research will have implications to address food security issues.

智力优势在陆地植物中，许多细胞和发育过程都需要对机械刺激的感知，如触觉、重力或渗透压。实现机械感知的一种方式是通过激活机械敏感通道，其允许离子响应于膜拉伸而穿过膜。该项目旨在使用模式开花植物拟南芥来研究已建立的机械敏感通道在受精过程中所起的作用，并确定它们的活性是如何调节的。此外，新的植物机械敏感通道将被确定为未来的功能和比较研究。这项研究将揭示植物感知和响应机械刺激的分子机制，深入了解机械敏感通道的进化，并建立适用于其他系统的机械生物学原理。更广泛的影响将开发离子通道生理学的探究式实验室课程和两个独特设计的暑期本科研究/推广经验，给本科生一个真实的研究经验，同时有助于本项目的研究目标。其他活动将扩大代表性不足的群体的参与，并通过围绕圣路易斯科学中心的新臭氧花园和地球日庆祝活动开展的既定外联活动，提高科学认识。贯穿所有这些活动的一个主题是有效的科学传播培训。此外，这项研究将通过解决植物生长，发育，生育，健康和应对压力的机制来造福社会。这项研究将对解决粮食安全问题产生影响。